Eng R H, Smith S M, Cherubin C E, Tan E N
Infectious Disease Section/Medical Service, Veterans Administration Medical Center, East Orange, New Jersey 07019.
Eur J Clin Microbiol Infect Dis. 1991 Jan;10(1):34-8. doi: 10.1007/BF01967095.
The antibiotic carry-over effect occurs when antibiotic transferred onto the agar plate with the subcultured aliquot is sufficient to inhibit the growth of viable bacteria, and results in a falsely low MBC. This phenomenon was eliminated by widely streaking the transferred aliquot over at least one half of a 100 mm agar plate or by centrifugation and resuspension of the organisms in non-antibiotic-containing media prior to plating. Both methods require more effort than the current method of MBC determination, but can be performed in clinical laboratories and are thus highly recommended in the testing of organisms from endocarditis and meningitis patients.
当转移到琼脂平板上的亚培养等分试样中的抗生素足以抑制活菌生长时，就会出现抗生素残留效应，从而导致最低杀菌浓度（MBC）被错误地低估。通过将转移的等分试样广泛涂布在至少半个100mm琼脂平板上，或在接种前将微生物离心并重悬于不含抗生素的培养基中，可消除这种现象。这两种方法都比目前测定MBC的方法需要更多的工作量，但可以在临床实验室中进行，因此在对心内膜炎和脑膜炎患者的病原体检测中强烈推荐使用。
