金黄色葡萄球菌对苯唑西林最低杀菌浓度的测定：技术因素的影响及意义
Determination of minimum bactericidal concentrations of oxacillin for Staphylococcus aureus: influence and significance of technical factors.
作者信息
Taylor P C, Schoenknecht F D, Sherris J C, Linner E C
出版信息
Antimicrob Agents Chemother. 1983 Jan;23(1):142-50. doi: 10.1128/AAC.23.1.142.
Abstract
The minimum bactericidal concentration of oxacillin for Staphylococcus aureus was shown to be considerably influenced by technical and definitional factors, particularly by the survival of some organisms on the walls of test tubes and by the growth phase of the inoculum. Attention to technical detail greatly improved reproducibility, and log-phase cultures of all strains showed greater than 99.9% killing in 24 h, at or close to the minimum inhibitory concentration, including eight strains described as tolerant. Some strains showed the paradoxical phenomenon of having more survivors in higher concentrations above the minimum bactericidal concentration. An accepted reference minimum bactericidal concentration procedure is needed for establishing clinical correlates and for a review of endpoint criteria. Routine minimum bactericidal concentration tests on S. aureus should be interpreted with great caution.
摘要
已表明，苯唑西林对金黄色葡萄球菌的最低杀菌浓度受技术和定义因素的显著影响，特别是受试管壁上某些微生物的存活情况以及接种物生长阶段的影响。注重技术细节可大大提高重现性，所有菌株的对数期培养物在24小时内，在或接近最低抑菌浓度时，显示出超过99.9%的杀菌率，其中包括8株被描述为耐受性的菌株。一些菌株表现出矛盾的现象，即在高于最低杀菌浓度的较高浓度下有更多的存活菌。需要一种公认的参考最低杀菌浓度程序来建立临床相关性并审查终点标准。对金黄色葡萄球菌进行常规最低杀菌浓度测试时应极其谨慎地解读。
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