Kulkhanova D S, Erst A A, Novikova T I
Ontogenez. 2015 Jul-Aug;46(4):259-66.
Features of in vitro regeneration of Fritillaria sonnikovae from bulb scales were studied. The initiation of shoot formation was obtained on a nutrient medium BDS, supplemented with 5 µM 6-benzylaminopurine and 2 µM α-naphthaleneacetic acid. Optimization of propagation stage was carried out using obtained microbulbs as explants. High regenerative response of explants and shoot multiplication rate were observed on both media supplemented with growth regulators (up to 47% and 4.2 ± 0.6 pcs./explant, respectively) and hormone-free medium (48% and 4.1 ± 0.2 shoots per explant, respectively). It has been established that the ad- dition of growth regulators on the stage of cultivation does not cause increased morphogenic response but contributes to the accelerated initiation and development of microbulbs. Morphological and histological analysis revealed the dynamics of the formation of shoots de novo. In vitro development of F. sonnikovae follows the path of direct organogenesis from the epidermal tissue of the explant.
研究了川贝母鳞茎鳞片离体再生的特征。在添加了5 μM 6-苄基腺嘌呤和2 μM α-萘乙酸的BDS营养培养基上获得了芽形成的起始。以获得的微型鳞茎为外植体进行增殖阶段的优化。在添加生长调节剂的培养基（分别高达47%和4.2±0.6个/外植体）和无激素培养基（分别为48%和4.1±0.2个芽/外植体）上均观察到外植体的高再生反应和芽增殖率。已经确定，在培养阶段添加生长调节剂不会导致形态发生反应增加，但有助于微型鳞茎的加速起始和发育。形态学和组织学分析揭示了从头形成芽的动态过程。川贝母的离体发育遵循从外植体表皮组织直接器官发生的途径。
