Kolber D L, Shultz L D, Rothstein T L
Department of Microbiology, Evans Memorial Department of Clinical Research, Boston University Medical Center, MA.
Eur J Immunol. 1991 Mar;21(3):721-9. doi: 10.1002/eji.1830210327.
Simultaneous analysis of stimulated cells for surface phenotype and DNA content by flow cytometry was used to delineate the characteristics of murine lymphocytes that respond to phorbol esters. Peritoneal cells that expressed high-density sIgM, Ly-1, Mac-1 and high-density Pgp-1, were preferentially stimulated to enter the S + G2/M phases of the cell cycle by the protein kinase C agonist, phorbol 12-myristate 13-acetate (PMA). Since these phenotypic characteristics correspond well with those of Ly-1-lineage B cells, non-peritoneal sources of Ly-1+ B cells were examined to test the relative role of lineage and environment in determining phorbol ester responsiveness. Splenic sIgM+ lymphocytes from adult mev/mev mice, and Ly-1+ splenic B cells from neonatal normal mice, were stimulated to enter the S + G2/M phases by PMA, although not to the same extent as peritoneal Ly-1+ B cells. These results suggest that responsiveness to phorbol ester, acting alone, is characteristic of murine Ly-1-lineage B cells regardless of tissue of origin; however, a role for environmental influences has not been completely ruled out.
采用流式细胞术同时分析受刺激细胞的表面表型和DNA含量，以描绘对佛波酯产生反应的小鼠淋巴细胞的特征。表达高密度sIgM、Ly-1、Mac-1和高密度Pgp-1的腹膜细胞，被蛋白激酶C激动剂佛波醇12-肉豆蔻酸酯13-乙酸酯（PMA）优先刺激进入细胞周期的S + G2/M期。由于这些表型特征与Ly-1谱系B细胞的特征非常吻合，因此对Ly-1 + B细胞的非腹膜来源进行了检测，以测试谱系和环境在决定佛波酯反应性中的相对作用。成年mev/mev小鼠的脾脏sIgM +淋巴细胞，以及新生正常小鼠的Ly-1 +脾脏B细胞，被PMA刺激进入S + G2/M期，尽管程度不如腹膜Ly-1 + B细胞。这些结果表明，单独作用时，对佛波酯的反应性是小鼠Ly-1谱系B细胞的特征，无论其起源组织如何；然而，环境影响的作用尚未完全排除。
