Rothstein T L, Kolber D L
Evans Memorial Department of Clinical Research, Boston University Medical Center, MA 02118.
J Immunol. 1988 Dec 15;141(12):4089-93.
Peritoneal B cells are stimulated to enter S phase by phorbol esters acting alone, in the absence of a co-mitogen. Anti-Ig antibody inhibited the stimulation of peritoneal B cells induced by phorbol esters and this inhibition was apparent throughout the time course of PMA-induced stimulation. Assessment of inhibition after depletion of Ly-1+ B cells suggested that PMA-induced stimulation of both Ly-1+ and Ly-1- B cells was affected by anti-Ig. Inhibition produced by anti-Ig was substantially reduced after even a brief delay in the addition of antibody to PMA-stimulated B cells. Anti-Ig antibody may counteract early events triggered by PMA within minutes of peritoneal B cell stimulation.
在没有共刺激原的情况下，佛波酯单独作用就能刺激腹膜B细胞进入S期。抗Ig抗体抑制了佛波酯诱导的腹膜B细胞刺激，并且在PMA诱导刺激的整个时间过程中这种抑制都很明显。对Ly-1+B细胞耗竭后的抑制作用评估表明，PMA诱导的Ly-1+和Ly-1-B细胞刺激均受到抗Ig的影响。在向PMA刺激的B细胞中添加抗体即使稍有延迟后，抗Ig产生的抑制作用也会大幅降低。抗Ig抗体可能在腹膜B细胞刺激后几分钟内抵消由PMA触发的早期事件。
