Murphy T P, Kolber D L, Rothstein T L
Evans Memorial Department of Clinical Research, Boston University Medical Center, MA.
Eur J Immunol. 1990 May;20(5):1137-42. doi: 10.1002/eji.1830200529.
B cell expression of the surface glycoprotein, Pgp-1 (Ly-24), was evaluated using flow cytometric analysis. Pgp-1 expression on naive, conventional (splenic) B cells was low but could be increased by mitogenic stimulation. Pgp-1 expression on naive peritoneal B cells was higher than expression by unmanipulated conventional B cells, suggesting the possibility that peritoneal B cells have been activated in vivo. However, the elevated expression of Pgp-1 by peritoneal B cells was not accompanied by increased expression of surface Ia but was correlated with Ly-1 status. Further, Pgp-1 expression by B cells from germ-free mice did not differ from that of normal animals. The elevated expression of Pgp-1 by peritoneal B cells is superficially similar to that provoked by mitogenic stimulation of conventional B cells; however, several characteristics suggest that the elevated expression of Pgp-1 by peritoneal B cells does not reflect prior activation of these cells.
使用流式细胞术分析评估表面糖蛋白Pgp-1（Ly-24）的B细胞表达。幼稚的常规（脾）B细胞上的Pgp-1表达较低，但可通过促有丝分裂刺激而增加。幼稚腹膜B细胞上的Pgp-1表达高于未处理的常规B细胞，提示腹膜B细胞可能已在体内被激活。然而，腹膜B细胞Pgp-1表达的升高并未伴随表面Ia表达的增加，而是与Ly-1状态相关。此外，无菌小鼠B细胞的Pgp-1表达与正常动物无异。腹膜B细胞Pgp-1表达的升高表面上类似于常规B细胞促有丝分裂刺激所引发的情况；然而，几个特征表明腹膜B细胞Pgp-1表达的升高并不反映这些细胞先前的激活。
