Rothstein T L, Kolber D L
Evans Memorial Department of Clinical Research, Boston University Medical Center, MA 02118.
J Immunol. 1988 May 1;140(9):2880-5.
B cells obtained by irrigation of the peritoneal cavity differ from splenic B cells in signaling requirements for the initiation of DNA synthesis. Splenic B cells are stimulated to enter S phase by phorbol esters in conjunction with a second signal provided by calcium ionophore; however, splenic B cells are not stimulated by phorbol ester alone. In contrast, peritoneal B cells from NZB and BALB/c mice were stimulated to incorporate tritiated thymidine by each of the phorbol esters, PMA and phorbol dibutyrate, acting alone. Stimulation of peritoneal B cells was apparent when cells were cultured at lower than usual cell densities, and responses were unaffected by coculture with splenic B cells. Responding cells adhered to plastic petri dishes coated with anti-mouse IgM antibody, but were not completely removed by treatment with anti-Ly-1.2 antibody plus C. These results indicate that phorbol esters constitute a complete signal that stimulates some peritoneal B cells to enter S phase.
通过腹腔灌洗获得的B细胞在启动DNA合成的信号需求方面与脾B细胞不同。佛波酯与钙离子载体提供的第二个信号共同作用可刺激脾B细胞进入S期；然而，单独的佛波酯并不能刺激脾B细胞。相比之下，来自NZB和BALB/c小鼠的腹腔B细胞可被佛波酯、PMA和佛波二丁酸单独作用刺激掺入氚标记的胸腺嘧啶核苷。当细胞以低于正常细胞密度培养时，腹腔B细胞的刺激很明显，并且与脾B细胞共培养时反应不受影响。反应细胞黏附于包被抗小鼠IgM抗体的塑料培养皿，但用抗Ly-1.2抗体加补体处理并不能将其完全去除。这些结果表明佛波酯构成了一个完整信号，可刺激一些腹腔B细胞进入S期。
