Slater M
Electron Microscope Unit, Prince Henry Hospital, Little Bay, Sydney, NSW, Australia.
Experientia. 1991 Mar 15;47(3):267-70. doi: 10.1007/BF01958156.
Normal human autopsy anterior pituitary tissue from 5 cases was embedded in LR White resin and immunolabelled using silver-enhanced 5-nm protein A gold probes. Follicle stimulating hormone (FSH), thyroid stimulating hormone (TSH), luteinizing hormone (LH), adrenocorticotrophic hormone (ACTH), growth hormone (GH) and prolactin (PRL) were immunolocalised to the level of secretory granule. A two-sided double-labelling method was used to cross-react two hormones at a time with respect to their corresponding antibodies. All possible combinations of the six pituitary hormones were tested. Plurihormonal granules were found that contained LH + FSH, LH + TSH, and FSH + TSH. Each hormone was also found in monohormonal granules. Granule diameter was significantly larger in the pluri as opposed to monohormonal granules.
取自5例正常人类尸检的垂体前叶组织被包埋于LR White树脂中，并使用银增强的5纳米蛋白A金探针进行免疫标记。促卵泡激素（FSH）、促甲状腺激素（TSH）、黄体生成素（LH）、促肾上腺皮质激素（ACTH）、生长激素（GH）和催乳素（PRL）被免疫定位到分泌颗粒水平。采用双侧双标记法，使两种激素同时与其相应抗体发生交叉反应。对六种垂体激素的所有可能组合进行了测试。发现了含有LH + FSH、LH + TSH和FSH + TSH的多激素颗粒。每种激素也存在于单激素颗粒中。与单激素颗粒相比，多激素颗粒的直径明显更大。
