Institute of Molecular Physiology and Genetics, Centre of Excellence for Cardiovascular Research, Slovak Academy of Sciences and Molecular Medicine Center, Slovak Academy of Sciences, 83334 Bratislava, Slovakia.
FEBS Lett. 2010 May 17;584(10):2085-92. doi: 10.1016/j.febslet.2010.01.035. Epub 2010 Jan 26.
This minireview focuses on observation of the properties, functional significance, and modulation of single chloride channels in the mitochondrial inner membrane using two electrophysiological methods--the patch-clamp and bilayer lipid membrane methods. Measurements of parameters such as conductance, Cl(-)/K(+) selectivity, voltage or pH dependence as well as their modulation by endogenous and exogenous compounds using individual mitochondrial chloride channels result in an unexpectedly wide range of values. This paper discusses the origin of this wide variety of channel parameters and the possible involvement of these channels in mitochondrial membrane potential oscillations, apoptosis, carrier function, and mitochondrial fusion and fission.
这篇小综述专注于使用两种电生理方法——膜片箝位和双层脂质膜方法——观察线粒体内膜中单氯离子通道的特性、功能意义和调节。使用单个线粒体氯离子通道测量电导、Cl(-)/K(+)选择性、电压或 pH 依赖性以及内源性和外源性化合物对其的调节等参数，结果显示出出乎意料的广泛范围的值。本文讨论了这种广泛的通道参数的起源以及这些通道可能参与线粒体膜电位振荡、细胞凋亡、载体功能以及线粒体融合和裂变。
