Department of Public Health, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Okayama, 700-8558, Japan.
Bull Environ Contam Toxicol. 2010 Mar;84(3):347-50. doi: 10.1007/s00128-010-9936-9. Epub 2010 Jan 26.
We evaluated pyrogallol cytotoxicity using Escherichia coli strains that express mammalian catalase gene derived from catalase mutant mice (Cs(b)) and wild-type (Cs(a)), and pyrogallol mutagenicity by Ames test. Pyrogallol was more toxic to Cs(b) rather than to Cs(a) (p < 0.05), while catalase, superoxide dismutase and ascorbic acid decrease the toxic effect. Pyrogallol also showed mutagenic effect (mutagenic index = 3.8 for 10 micromol pyrogallol/plate) while ascorbic acid (19.4% reduction, p < 0.001) and naringin (35.1% reduction, p < 0.001) played a protective role against it. Pyrogallol cytotoxicity and mutagenicity seem to be attributable, at least in part, to reactive oxygen species formation. This study also suggests that newly established catalase mutant E. coli is probably useful in hazard identification of oxidative chemicals.
我们使用表达来源于过氧化氢酶突变小鼠（Cs(b)）和野生型（Cs(a)）的哺乳动物过氧化氢酶基因的大肠杆菌菌株来评估焦儿茶酚的细胞毒性，并通过艾姆斯试验来评估焦儿茶酚的诱变作用。焦儿茶酚对 Cs(b)的毒性比 Cs(a)大（p<0.05），而过氧化氢酶、超氧化物歧化酶和抗坏血酸则降低了毒性作用。焦儿茶酚还具有诱变作用（10 μmol 焦儿茶酚/平板的诱变指数为 3.8），而抗坏血酸（降低 19.4%，p<0.001）和柚皮苷（降低 35.1%，p<0.001）则对其起到保护作用。焦儿茶酚的细胞毒性和诱变作用似乎至少部分归因于活性氧的形成。本研究还表明，新建立的过氧化氢酶突变大肠杆菌菌株可能有助于识别氧化应激化学品的危害。
