Parent R A, Caravello H E, San R H
Consultox Ltd., Damariscotta, ME 04543, USA.
J Appl Toxicol. 1996 Mar-Apr;16(2):103-8. doi: 10.1002/(SICI)1099-1263(199603)16:2<103::AID-JAT318>3.0.CO;2-Q.
Acrolein was tested for mutagenic activity in seven strains of Salmonella typhimurium and one strain of Escherichia coli using a preincubation assay procedure. Cytotoxicity was evident at dosing levels above 33 and 67 micrograms acrolein per plate in the absence and presence of S-9 activation, respectively. Evidence of mutagenic activity was seen at non-toxic dosing levels in S. typhimurium strains TA98 and TA100 and E. coli strain WP2 uvrA. Responses in TA98 and E. coli were marginal at best, but a firm positive mutagenic activity was noted in TA100 at 20 micrograms per plate without S-9 activation and at 67 micrograms per plate with S-9 activation. The results of this study demonstrate the mutagenicity of acrolein under highly controlled conditions.
采用预培养试验程序，在七种鼠伤寒沙门氏菌菌株和一种大肠杆菌菌株中对丙烯醛的致突变活性进行了测试。在不存在和存在S - 9活化的情况下，当每平板丙烯醛给药水平分别高于33微克和67微克时，细胞毒性明显。在鼠伤寒沙门氏菌TA98和TA100菌株以及大肠杆菌WP2 uvrA菌株中，在无毒给药水平下观察到了致突变活性的证据。TA98和大肠杆菌中的反应充其量只是微弱的，但在TA100中，在无S - 9活化时每平板20微克以及有S - 9活化时每平板67微克的情况下，观察到了确凿的阳性致突变活性。本研究结果证明了在高度受控条件下丙烯醛的致突变性。
