Arnold W R, Diamond R L, Smith D S
Aquatic Ecological Risk Assessment LLC, 4411 Kinsey Drive, No. 635, Tyler, TX 75703, USA.
Arch Environ Contam Toxicol. 2010 Aug;59(2):225-34. doi: 10.1007/s00244-010-9467-8. Epub 2010 Jan 26.
This paper presents data from original research for use in the development of a marine biotic ligand model and, ultimately, copper criteria for the protection of estuarine and marine organisms and their uses. Ten 48-h static acute (unfed) copper toxicity tests using the euryhaline rotifer Brachionus plicatilis ("L" strain) were performed to assess the effects of salinity, pH, and dissolved organic matter (measured as dissolved organic carbon; DOC) on median lethal dissolved copper concentrations (LC50). Reconstituted and natural saltwater samples were tested at seven salinities (6, 11, 13, 15, 20, 24, and 29 g/L), over a pH range of 6.8-8.6 and a range of dissolved organic carbon of <0.5-4.1 mg C/L. Water chemistry analyses (alkalinity, calcium, chloride, DOC, hardness, magnesium, potassium, sodium, salinity, and temperature) are presented for input parameters to the biotic ligand model. In stepwise multiple regression analysis of experimental results where salinity, pH, and DOC concentrations varied, copper toxicity was significantly related only to the dissolved organic matter content (pH and salinity not statistically retained; alpha=0.05). The relationship of the 48-h dissolved copper LC50 values and dissolved organic carbon concentrations was LC50 (microg Cu/L)=27.1xDOC (mg C/L)1.25; r2=0.94.
本文展示了来自原始研究的数据，用于开发海洋生物配体模型，并最终制定保护河口和海洋生物的铜标准及其应用。使用广盐性轮虫褶皱臂尾轮虫（“L”品系）进行了10次48小时静态急性（未投喂）铜毒性试验，以评估盐度、pH值和溶解有机物（以溶解有机碳；DOC衡量）对半数致死溶解铜浓度（LC50）的影响。在七种盐度（6、11、13、15、20、24和29 g/L）、pH值范围为6.8 - 8.6以及溶解有机碳范围为<0.5 - 4.1 mg C/L的条件下，对重构海水和天然海水样本进行了测试。给出了生物配体模型输入参数的水化学分析（碱度、钙、氯化物、DOC、硬度、镁、钾、钠、盐度和温度）结果。在盐度、pH值和DOC浓度变化的实验结果逐步多元回归分析中，铜毒性仅与溶解有机物含量显著相关（pH值和盐度未被统计学保留；α = 0.05）。48小时溶解铜LC50值与溶解有机碳浓度的关系为LC50（μg Cu/L）= 27.1×DOC（mg C/L）^1.25；r2 = 0.94。
