Wietholtz H, Marschall H U, Reuschenbach R, Matern H, Matern S
Medizinische Klinik III, Aachen University of Technology, Germany.
Hepatology. 1991 Apr;13(4):656-62.
Recently the formation of bile acid glucosides has been described as a novel conjugation mechanism in vitro and in vivo. In 10 patients with extrahepatic cholestasis caused by carcinoma of the head of the pancreas we investigated excretion rates and profiles of urinary bile acid glucosides. Urinary bile acid glucosides and, for comparison, bile acid glucuronides were extracted and characterized according to established methods. In controls total urinary bile acid glucoside excretion was 0.22 +/- 0.03 mumol/24 hr (mean +/- S.E.M.)-in the range of bile acid glucuronide excretion (0.41 +/- 0.06 mumol/24 hr; mean +/- S.E.M.). A gas chromatography-mass spectrometry-characterized trihydroxy bile acid glucoside of still-unknown hydroxyl positions accounted for 65% of total urinary bile acid glucosides. In extrahepatic cholestasis total urinary bile acid glucoside excretion was 0.52 +/- 0.13 mumol/24 hr (mean +/- SEM), yet significantly lower than bile acid glucuronide excretion (1.53 +/- 0.13 mumol/24 hr; mean +/- SEM; p less than 0.001). In cholestasis the primary bile acid derivatives cholic and chenodeoxycholic acid glucosides amounted to 90%, whereas the trihydroxy bile acid glucoside had decreased to 5% of total bile acid glucoside excretion, indicating its alteration during enterohepatic circulation. The data establish the composition and quantity of urinary bile acid glucosides in healthy controls and cholestasis and constitute a quantitative comparison with another glycosidic conjugation reaction, bile acid glucuronidation.
最近，胆汁酸葡糖苷的形成被描述为一种在体外和体内的新型结合机制。我们研究了10例由胰头癌引起的肝外胆汁淤积患者尿中胆汁酸葡糖苷的排泄率和排泄谱。根据既定方法提取并鉴定了尿中胆汁酸葡糖苷以及作为对照的胆汁酸葡糖醛酸苷。在对照组中，尿中胆汁酸葡糖苷的总排泄量为0.22±0.03μmol/24小时（平均值±标准误），处于胆汁酸葡糖醛酸苷排泄量（0.41±0.06μmol/24小时；平均值±标准误）范围内。一种羟基位置仍未知的经气相色谱 - 质谱法鉴定的三羟基胆汁酸葡糖苷占尿中胆汁酸葡糖苷总量的65%。在肝外胆汁淤积患者中，尿中胆汁酸葡糖苷的总排泄量为0.52±0.13μmol/24小时（平均值±标准误），但显著低于胆汁酸葡糖醛酸苷的排泄量（1.53±0.13μmol/24小时；平均值±标准误；p<0.001）。在胆汁淤积时，初级胆汁酸衍生物胆酸和鹅去氧胆酸葡糖苷占胆汁酸葡糖苷总排泄量的90%，而三羟基胆汁酸葡糖苷已降至胆汁酸葡糖苷总排泄量的5%，表明其在肠肝循环过程中发生了改变。这些数据确定了健康对照者和胆汁淤积患者尿中胆汁酸葡糖苷的组成和数量，并与另一种糖苷结合反应——胆汁酸葡糖醛酸化构成了定量比较。
