Kondi-Pafiti A, Frangou-Plemmenou M, Bakalianou C, Tsantopoulos M, Papadias K, Liapis A
Department of Pathology, Pathology Laboratory, University of Athens, Aretaieion Hospital, Athens, Greece.
Clin Exp Obstet Gynecol. 2009;36(4):226-9.
The stroma often fibroepithelial and ten adenomatous cervical polyps, 12 fibro-epithelial vaginal polyps, 20 non neoplastic cervices, 15 cone specimens with CIN changes and five specimens with infiltrative squamous cell carcinoma was investigated. Vimentin, desmin, actin, collagen III, CD34, ER and PgR were studied using the Ventana Automatic Immunostaining System. A subepithelial stromal layer was detected in 17/20 normal specimens, 16/20 cervical polyps, 10/12 vaginal polyps and in 6/15 cone biopsies but not in neoplastic specimens. Vimentin staining was positive in all fibroepithelial polyps, normal specimens and in 6/10 adenomatous polyps. Actin was negative in the loose subepithelial zone but positive in the underlying stroma. Desmin, alpha-1-antitrypsin and CD34 were detected in isolated cells in normal specimens. ER and PgR were observed in isolated subepithelial cells, with greater density in the cells of the fibromuscular wall. In conclusion, loose mesenchymal stroma in the lower female genital tract of a fibrohistiocytic nature was observed with minimal smooth muscle participation--which is the matrix of vaginal and cervical polyps.
对10个纤维上皮性和10个腺瘤性宫颈息肉、12个纤维上皮性阴道息肉、20个非肿瘤性宫颈、15个有CIN改变的锥形标本以及5个浸润性鳞状细胞癌标本的基质进行了研究。使用Ventana自动免疫染色系统研究波形蛋白、结蛋白、肌动蛋白、胶原蛋白III、CD34、雌激素受体（ER）和孕激素受体（PgR）。在20个正常标本中的17个、20个宫颈息肉中的16个、12个阴道息肉中的10个以及15个锥形活检标本中的6个检测到上皮下基质层，但在肿瘤标本中未检测到。波形蛋白染色在所有纤维上皮性息肉、正常标本以及10个腺瘤性息肉中的6个呈阳性。肌动蛋白在疏松的上皮下区域呈阴性，但在其下方的基质中呈阳性。在正常标本的孤立细胞中检测到结蛋白、α-1抗胰蛋白酶和CD34。在孤立的上皮下细胞中观察到ER和PgR，在纤维肌壁的细胞中密度更高。总之，在女性下生殖道观察到具有纤维组织细胞性质的疏松间充质基质，平滑肌参与极少，这是阴道和宫颈息肉的基质。
