Benhamou P H, Kalach N, Soulaines P, Donne N, Dupont C
Université Paris Descartes and Department of Pediatrics-Neonatology, Pediatric Gastroenterology & Nutrition Unit, Cochin-Saint Vincent de Paul Hospital, 82 Avenue Denfert-Rochereau, 75674 Paris Cedex 14, France.
Eur Ann Allergy Clin Immunol. 2009 Oct;41(5):146-51.
to assess the accuracy and safety of a ready-to-use atopy patch test (HDM-Diallertest, DBV Technologies, Paris) in the diagnosis of sensitization to house-dust mite (HDM) allergens in children with or without atopic dermatitis.
prospective analysis of a systematic allergic work-up was carried out in 47 children, age 57.4+42 months (mean + SD, range 7 to 176 mo), presenting with isolated or combined atopic dermatitis (AD, n = 28) or other symptoms without AD (control group, n = 19). Children were routinely tested for specific HDM-IgE [against D. pteronyssinus (DPT) and D. farinae (DF)], and skin testing based on HDM (DPT & DF) skin prick test (SPT) and ready-to-use HDM-ATP (HDM-Diallertest), with a reading at 72 hours.
15 children (31.9%) exhibited specific IgE against both DPT and DF, 16 children (34.04%) exhibited positive SPT against DPT and 17 (36.1%) against DF. HDM-Diallertest was positive in 15 cases (31.9%). Among these, 9 exhibited with an eczematous reaction showed an excellent correlation with both SPT and specific IgE for DPT and DF, respectively 93.3%, 97.77%, 90.47%, and 90.47%. The different diagnostic techniques of HDM sensitization neither differ between groups, nor correlated specifically with the different clinical manifestations. No side effect was observed during and after patch testing, except for a local reaction without diffusion outside the local test area.
The 3 diagnostic techniques exhibited a comparable level of accuracy for the diagnosis of HDM allergens sensitization. The excellent concordance of the highest class reactions of HDM-Diallertest with the other diagnostic techniques indicates a potential role as a screening tool for the detection of HDM sensitization in infancy.
评估即用型特应性皮炎斑贴试验（HDM - Diallertest，DBV Technologies公司，巴黎）在诊断有或无特应性皮炎儿童对屋尘螨（HDM）过敏原致敏方面的准确性和安全性。
对47名儿童进行了系统性过敏检查的前瞻性分析，这些儿童年龄为57.4±42个月（平均±标准差，范围7至176个月），患有孤立性或合并性特应性皮炎（AD，n = 28）或无AD的其他症状（对照组，n = 19）。对儿童常规检测针对HDM的特异性IgE [针对粉尘螨（DPT）和屋尘螨（DF）]，并基于HDM（DPT和DF）进行皮肤点刺试验（SPT）和即用型HDM - ATP（HDM - Diallertest），72小时后读取结果。
15名儿童（31.9%）对DPT和DF均表现出特异性IgE，16名儿童（34.04%）对DPT的SPT呈阳性，17名儿童（36.1%）对DF的SPT呈阳性。HDM - Diallertest在15例（31.9%）中呈阳性。其中，9例出现湿疹样反应的患者分别与针对DPT和DF的SPT及特异性IgE表现出极好的相关性，分别为93.3%、97.77%、90.47%和90.47%。HDM致敏的不同诊断技术在各组之间无差异，也未与不同临床表现有特异性关联。除局部试验区域外无扩散的局部反应外，斑贴试验期间及之后未观察到副作用。
这三种诊断技术在诊断HDM过敏原致敏方面表现出相当的准确性水平。HDM - Diallertest最高等级反应与其他诊断技术的极好一致性表明其作为婴儿期HDM致敏检测筛查工具的潜在作用。
