Research Centre for the Characterization and Safe Use of Natural Compounds-Giovanni Galli, University of Milano, Via Balzaretti, 9, 20133 Milano, Italy.
J Agric Food Chem. 2010 Feb 24;58(4):2246-52. doi: 10.1021/jf9042503.
In vivo studies suggest that the phenolic component contributes to the anti-inflammatory and antiatherosclerotic actions of olive oil; however, the effects in circulating cells are not fully characterized. Monocytes play a key role in inflammation-based diseases by expressing several molecules, including metalloproteinases (MMPs). In the present study, we investigated the effects of olive oil phenolic extract and individual compounds on MMP-9 in THP-1 cells, a human monocyte-like cell line. Olive oil extract prevented the stimulation of MMP-9 expression and secretion in tumor necrosis factor alpha-treated THP-1 cells. Oleuropein aglycone, a typical olive oil phenol, was active at concentrations found in the extract, although other compounds probably contribute to the biological activity. We also found that the effect of the extract and individual compounds on MMP-9 is due to impaired nuclear factor-kappaB signaling. Our findings provide further evidence on the mechanisms by which olive oil reduces the inflammatory burden associated with disorders, such as atherosclerosis.
体内研究表明，酚类成分有助于橄榄油的抗炎和抗动脉粥样硬化作用；然而，其在循环细胞中的作用尚未完全阐明。单核细胞通过表达多种分子，包括金属蛋白酶（MMPs），在炎症相关疾病中发挥关键作用。在本研究中，我们研究了橄榄油酚提取物和单个化合物对人单核细胞样细胞系 THP-1 细胞中 MMP-9 的影响。橄榄油提取物可防止肿瘤坏死因子 α 处理的 THP-1 细胞中 MMP-9 表达和分泌的刺激。橄榄苦苷配基是一种典型的橄榄油酚，在提取物中存在的浓度下具有活性，尽管其他化合物可能有助于其生物活性。我们还发现，提取物和单个化合物对 MMP-9 的作用是由于核因子-κB 信号转导受损。我们的研究结果为橄榄油减轻与动脉粥样硬化等疾病相关的炎症负担的机制提供了进一步的证据。
