Urbani Lorenza, Tirolo Alessandro, Balboni Andrea, Troia Roberta, Dondi Francesco, Battilani Mara
Department of Veterinary Medical Sciences, Alma Mater Studiorum-University of Bologna, Bologna, Italy.
Front Vet Sci. 2022 Jul 19;9:964177. doi: 10.3389/fvets.2022.964177. eCollection 2022.
In this report the concomitant infection with canine parvovirus type 2 (CPV-2), and in two puppy dogs from Southern Italy is described. Dogs were referred to a veterinary university hospital for the acute onset of lethargy and gastrointestinal signs. A complete clinical and clinicopathological evaluation was carried out and the multiple infection was confirmed by microscopic detection of inclusion bodies in peripheral blood smear, rapid immunoenzymatic tests, indirect fluorescent antibody tests, and molecular assays. Sequence analysis revealed that the CPV-2 identified belonged to the 2c variant and had amino acid residues in the predicted VP2 protein typical of "Asian-like" strains widespread in Asia and occasionally reported in Romania, Nigeria and Italy, particularly in the region of Sicily. Numerous monocytes were infected by both gamonts and morulae, suggesting that this co-infection is not accidental and that preferably infects those cells parasitized by . The clinical presentation of these animals was severe but supportive cares associated with early etiological therapy allowed a good prognosis. Movement of puppies from geographic areas where vector-borne pathogens are endemic must be carefully evaluated and core vaccinations and ectoparasite prevention treatments must be rigorously adopted.
在本报告中，描述了意大利南部两只幼犬感染犬细小病毒2型（CPV-2）的情况。这些犬因突然出现嗜睡和胃肠道症状被转诊至一家兽医大学医院。进行了全面的临床和临床病理评估，并通过在外周血涂片中显微镜检测包涵体、快速免疫酶试验、间接荧光抗体试验和分子检测来确诊多重感染。序列分析显示，鉴定出的CPV-2属于2c变异株，其预测的VP2蛋白中的氨基酸残基具有在亚洲广泛传播且偶尔在罗马尼亚、尼日利亚和意大利（特别是西西里岛地区）报道的“亚洲样”毒株的典型特征。大量单核细胞被配子体和桑葚体感染，这表明这种共同感染并非偶然，且优先感染被[此处原文缺失相关寄生虫名称]寄生的细胞。这些动物的临床表现严重，但早期病因治疗结合支持性护理预后良好。必须仔细评估来自媒介传播病原体流行地区的幼犬的活动情况，并且必须严格采用核心疫苗接种和体外寄生虫预防治疗措施。
