[从一名死于肺炎的婴儿中分离出的腺病毒Ad7d2毒株的基因分析]

[Genetic analysis of a strain of adenovirus Ad7d2 isolated from an infant died of pneumonia].

作者信息

Tang Liu-ying, Liu Xiu-yun, Xu Wen-bo

机构信息

Institute for Viral Diseases Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100050, China.

出版信息

Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2009 Apr;23(2):127-8.

PMID:20104756

Abstract

OBJECTIVE

To analyze the genetic characteristics of a strain of adenovirus, Ad7d2, isolated from an infant died of severe pneumonia.

METHODS

Virus isolation was performed by using the nasopharyngeal secretion from an 11-month-old infant with serious pneumonia. The viral DNA was amplified by PCR and the products were sequenced.

RESULTS

One strain of virus was isolated and was named as BJ060316-1. Sequence analysis of the hexon and fiber gene of the PCR products showed that the strain was Ad7d2, which shared 99.5% homology for 950bp hexon fragment with AF321311 Ad7d2 isolated from Israel in 1993. Blast with deduced amino acid sequence showed that BJ060316-1 lost glutamine at site 253, and at the site 495 arginine replaced serine. For fiber gene, BJ060316-1 showed 99.7% homology with AB243118 Ad7 isolated in Japan in 2005 for 975 bp fragment.

CONCLUSION

Adenovirus Ad7d2 strain BJ060316-1 isolated from a an infant with fatal pneumonia showed no virulence mutation.

摘要

目的

分析从一名死于重症肺炎的婴儿体内分离出的一株腺病毒Ad7d2的基因特征。

方法

使用一名患有严重肺炎的11个月大婴儿的鼻咽分泌物进行病毒分离。通过PCR扩增病毒DNA并对产物进行测序。

结果

分离出一株病毒，命名为BJ060316 - 1。对PCR产物的六邻体和纤维基因进行序列分析表明，该毒株为Ad7d2，其950bp六邻体片段与1993年从以色列分离的AF321311 Ad7d2的同源性为99.5%。对推导的氨基酸序列进行比对显示，BJ060316 - 1在253位点缺失谷氨酰胺，在495位点精氨酸取代丝氨酸。对于纤维基因，BJ060316 - 1与2005年在日本分离的AB243118 Ad7的975bp片段同源性为99.7%。