Huang Guo-Yong, Wang Hai-Hong, Chen Xiao-Hua, Zhang Xiao-Mei, Tu Yan-Chai, Li Jian-Dao, Zhang Qiong, Chen Tong, Ge Sheng-Xiang
Wenzhou blood center, Wenzhou 325001, China.
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2009 Jun;23(3):185-7.
To investigate the southern region of Zhejiang hepatitis E virus (HEV) infection.
A cluster sampling strategy was used to sample all blood donors from February to October in 2008 in Wenzhou blood center. Their blood was tested for IgG and IgM antibody against HEV. Reverse transcriptase-polymerase chain reaction(RT-PCR) and sequencing were applied to detect its genotype and sequence homology in HEV IgM-positive specimen.
The prevalence of anti-HEV IgG in 3044 cases of blood donors was 33.28%. IgG increased with age. There are certain increase in positive rates between the 20-year-old group and over 40 years of age group from 21.16% to 50.36%. The positive rate of IgM was 0.92%. The ratio of infection among different age group was the highest in the age range from 31 to 40 years and up to 1.90%. IgG and IgM through their negative and positive analysis of samples found in their group with donors age, sex and blood type does not significantly related to each other. Nucleic acids were found in three cases through PCR amplification in all 28 cases of HEV IgM positive samples. The total positive rate was one-thousandth, of which two cases for gene 4, 1 cases of infection for gene 1.
The results indicate that there was a certain percentage of HEV virus in voluntary blood donors in south Zhejiang.
调查浙江南部地区戊型肝炎病毒（HEV）感染情况。
采用整群抽样策略，对2008年2月至10月温州血站的所有献血者进行采样。检测他们血液中的抗HEV IgG和IgM抗体。对HEV IgM阳性标本应用逆转录聚合酶链反应（RT-PCR）及测序方法检测其基因型和序列同源性。
3044例献血者中抗HEV IgG的阳性率为33.28%。IgG随年龄增长而升高。20岁组与40岁以上组阳性率有一定升高，从21.16%升至50.36%。IgM阳性率为0.92%。不同年龄组感染率在31至40岁年龄段最高，达1.90%。通过对样本IgG和IgM阴阳性分析发现，其在献血者组内与年龄、性别及血型之间无明显相关性。在所有28例HEV IgM阳性样本中，通过PCR扩增有3例检测到核酸。总阳性率为千分之一，其中2例为基因4型，1例为基因1型感染。
结果表明浙南地区无偿献血者中存在一定比例的HEV病毒感染。
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