Ikezono Tetsuo, Sugizaki Kazuki, Shindo Susumu, Sekiguchi Satomi, Pawankar Ruby, Baba Shunkichi, Yagi Toshiaki
Department of Otorhinolaryngology, Nippon Medical School, Tokyo, Japan.
Acta Otolaryngol. 2010 Aug;130(8):881-7. doi: 10.3109/00016480903508910.
By testing 125 samples, we confirmed that Cochlin-tomoprotein (CTP) is present in the perilymph, not in cerebrospinal fluid (CSF). Perilymph and CSF exist in two distinct compartments, even in the case of a malformed inner ear with a bony defect in the lamina cribrosa, as described here. Cochleostomy might have suddenly decreased the perilymph pressure, allowing the influx of CSF into the inner ear resulting in profuse fluid leakage, first perilymph then CSF.
The first purpose of this study was to further confirm the specificity of the perilymph-specific protein CTP that we reported recently. Secondly, we assessed the nature of the fluid leakage from the cochleostomy using the CTP detection test.
A standardized CTP detection test was performed on 65 perilymph and 60 CSF samples. Samples of profuse fluid leakage collected from cochleostomy during cochlear implantation surgery of one patient with branchio-oto-renal (BOR) syndrome were also tested by the CTP detection test.
CTP was detected in 60 of 65 perilymph samples but not in any of the CSF samples. The leaked fluid was shown to contain CTP, i.e. perilymph, at the outset, and then the CTP detection signals gradually disappeared as time elapsed.
通过检测125份样本，我们证实了耳蜗脂蛋白（CTP）存在于外淋巴中，而非脑脊液（CSF）中。即使在内耳畸形且筛板存在骨质缺损的情况下，外淋巴和脑脊液也存在于两个不同的腔室中，如此处所述。耳蜗造瘘术可能突然降低了外淋巴压力，使得脑脊液流入内耳，导致大量液体渗漏，先是外淋巴，然后是脑脊液。
本研究的首要目的是进一步证实我们最近报道的外淋巴特异性蛋白CTP的特异性。其次，我们使用CTP检测试验评估了耳蜗造瘘术漏出液体的性质。
对65份外淋巴样本和60份脑脊液样本进行了标准化的CTP检测试验。对1例鳃-耳-肾（BOR）综合征患者在人工耳蜗植入手术期间从耳蜗造瘘术收集的大量漏出液样本也进行了CTP检测试验。
65份外淋巴样本中的60份检测到CTP，但脑脊液样本中均未检测到。结果显示，漏出液一开始含有CTP，即外淋巴，然后随着时间推移，CTP检测信号逐渐消失。
