Department of Health Sciences, Discipline of Oral and Maxillofacial Surgery, School of Dentistry, Sagrado Coração University, USC, Bauru, Sao Paulo, SP, Brazil.
Clin Oral Implants Res. 2010 Jun;21(6):584-90. doi: 10.1111/j.1600-0501.2009.01858.x. Epub 2010 Jan 23.
To analyze and compare the expression of core binding factor-1 (Cbfa-1)/Runx2 and vascular endothelium growth factor (VEGF) in sinus lift procedures using bovine hydroxyapatite (HA) and beta-tricalcium phosphate (beta-TCP).
Twenty-four male rabbits that had undergone bilateral sinus lift procedures were divided into three groups, according to the sinus filling material: Group 1: autogenous bone graft; Group 2: bovine HA; and Group 3: beta-TCP. All groups were sacrificed after 7, 14, 30 and 60 days, for microscopic, histomorphometry and immunohistochemistry analysis.
Microscopic analysis showed a similar bone repair pattern between the tested groups. New bone formation, soft and medular tissue, remaining material or particulate bone graft area were obtained by histomorphometric analysis. After 14 days, statistically significant differences in new bone formation were found between Group 1 (27.76+/-7.8) and Groups 2 (14.22+/-3.2) and 3 (11.1+/-7.7). After 30 days, statistically significant differences (P<0.05) were detected in bone formation between Groups 1 (31.39+/-36.5) and 2 (14.13+/-3.2). The last period showed improved bone formation in Group 2. Also, Group 2 showed higher Cbfa-1/Runx2 immunoexpression when compared with Group 3. No remarkable differences were observed in VEGF immunoexpression among groups.
Taken together, both biomaterials allowed bone tissue growth in a conductive pattern and did not interfere with bone remodeling in the late period, with a slight improvement in bone tissue formation when using HA, confirmed by marked expression of Cbfa-1 at initial periods.
分析和比较使用牛羟磷灰石（HA）和β-磷酸三钙（β-TCP）进行窦提升术时核心结合因子-1（Cbfa-1）/Runx2 和血管内皮生长因子（VEGF）的表达。
24 只雄性兔接受双侧窦提升术，根据窦填充材料分为三组：第 1 组：自体骨移植；第 2 组：牛 HA；第 3 组：β-TCP。所有组在第 7、14、30 和 60 天处死，进行显微镜、组织形态计量和免疫组织化学分析。
显微镜分析显示各组具有相似的骨修复模式。通过组织形态计量分析获得新骨形成、软、骨髓组织、剩余材料或颗粒状骨移植物区域。第 14 天，第 1 组（27.76+/-7.8）与第 2 组（14.22+/-3.2）和第 3 组（11.1+/-7.7）的新骨形成有统计学显著差异。第 30 天，第 1 组（31.39+/-36.5）和第 2 组（14.13+/-3.2）之间的骨形成有统计学显著差异（P<0.05）。最后一个时期，第 2 组的骨形成得到改善。此外，与第 3 组相比，第 2 组的 Cbfa-1/Runx2 免疫表达更高。各组之间的 VEGF 免疫表达无显著差异。
综上所述，两种生物材料均允许在引导模式下生长骨组织，并且在后期不会干扰骨重塑，使用 HA 时略有改善骨组织形成，初期 Cbfa-1 表达明显。
