Amarilla Alberto A, de Almeida Flavia T, Jorge Daniel M, Alfonso Helda L, de Castro-Jorge Luiza A, Nogueira Nadia A, Figueiredo Luiz T, Aquino Victor H
Virology Research Center, School of Medicine of Ribeirão Preto/USP, Ribeirão Preto - SP, Brazil.
Virol J. 2009 Jul 23;6:113. doi: 10.1186/1743-422X-6-113.
Dengue is the most important arbovirus disease in tropical and subtropical countries. The viral envelope (E) protein is responsible for cell receptor binding and is the main target of neutralizing antibodies. The aim of this study was to analyze the diversity of the E protein gene of DENV-3. E protein gene sequences of 20 new viruses isolated in Ribeirao Preto, Brazil, and 427 sequences retrieved from GenBank were aligned for diversity and phylogenetic analysis.
Comparison of the E protein gene sequences revealed the presence of 47 variable sites distributed in the protein; most of those amino acids changes are located on the viral surface. The phylogenetic analysis showed the distribution of DENV-3 in four genotypes. Genotypes I, II and III revealed internal groups that we have called lineages and sub-lineages. All amino acids that characterize a group (genotype, lineage, or sub-lineage) are located in the 47 variable sites of the E protein.
Our results provide information about the most frequent amino acid changes and diversity of the E protein of DENV-3.
登革热是热带和亚热带国家最重要的虫媒病毒病。病毒包膜（E）蛋白负责细胞受体结合，是中和抗体的主要靶点。本研究的目的是分析登革热病毒3型（DENV-3）E蛋白基因的多样性。对在巴西里贝朗普雷图分离的20株新病毒的E蛋白基因序列以及从GenBank检索到的427条序列进行比对，以进行多样性和系统发育分析。
E蛋白基因序列比较显示，该蛋白存在47个可变位点；这些氨基酸变化大多位于病毒表面。系统发育分析显示DENV-3分布于四种基因型中。基因型I、II和III显示出我们称为谱系和亚谱系的内部群体。表征一个群体（基因型、谱系或亚谱系）的所有氨基酸都位于E蛋白的47个可变位点中。
我们的结果提供了有关DENV-3 E蛋白最常见氨基酸变化和多样性的信息。
