Department of Pathological Biochemistry, Otto-von-Guericke University, Magdeburg, Germany.
Phytomedicine. 2010 Jul;17(8-9):589-97. doi: 10.1016/j.phymed.2009.12.002. Epub 2010 Jan 27.
At low concentration H(2)O(2) is an important signal molecule in proliferation of tumour cells. We report about a study investigating the effect of an ethanolic extract from Gynostemma pentaphyllum on proliferation of C6 glioma tumour cells and cellular H(2)O(2) concentration. The proliferation of these cells was maximal at about 1 muM extracellular H(2)O(2). HPLC-finger prints of the extract revealed a set of saponines as essential components. In C6 glioma cells the extract caused increase in super oxide dismutase (SOD) activity, in the amount of SOD protein, and in cellular H(2)O(2) concentration. It inhibited cell proliferation and induced activation of caspase 3 as indication of apoptosis. No effect of the extract was observed on the proliferation of astrocytes of a primary cell culture. From these findings we suggest that the ethanolic extract from Gynostemma pentaphyllum may selectively shift the H(2)O(2) concentration to toxic levels exclusively in tumour cells due to increased SOD activity. It may have a high potency in cancer therapy and cancer prophylaxis.
低浓度的 H(2)O(2)是肿瘤细胞增殖过程中的一个重要信号分子。我们报告了一项研究，该研究调查了绞股蓝乙醇提取物对 C6 神经胶质瘤肿瘤细胞增殖和细胞内 H(2)O(2)浓度的影响。这些细胞的增殖在约 1 μM 细胞外 H(2)O(2)时达到最大值。提取物的 HPLC 指纹图谱显示了一组皂甙作为必需成分。在 C6 神经胶质瘤细胞中，提取物导致超氧化物歧化酶 (SOD) 活性增加、SOD 蛋白含量增加以及细胞内 H(2)O(2)浓度增加。它抑制细胞增殖并诱导 caspase 3 的激活，表明发生了细胞凋亡。提取物对原代细胞培养的星形胶质细胞的增殖没有影响。根据这些发现，我们认为绞股蓝乙醇提取物可能由于 SOD 活性的增加，将 H(2)O(2)浓度选择性地转移到肿瘤细胞的毒性水平。它在癌症治疗和癌症预防方面可能具有很高的潜力。
