College of Veterinary Medicine, Hunan Agricultural University, Changsha, 410128, PR China.
Toxicol Mech Methods. 2010 Mar;20(3):153-8. doi: 10.3109/15376511003660185.
The objective of this study was to explore the effects of Gynostemma pentaphyllum on Zearalenone-induced apoptosis in mouse male germ cells. Fifty Kunming male mice at 25-days-old were classified into five groups: group A was the control (10% ethanol, 0.5 ml/day); group B with 10 microg Zearalenone/day; group C with 10 microg Zearalenone and 50 mg/kg/day Gynostemma pentaphyllum; group D with 10 microg Zearalenone and 100 mg/kg/day Gynostemma pentaphyllum; and group E with 10 microg Zearalenone and 200 mg/kg/day Gynostemma pentaphyllum. It was found that Gynostemma pentaphyllum has a marked effect on protecting male germ cells against Zearalenone-induced apoptosis, as evidenced by a reduced apoptosis rate of male germ cells and Bax expression as well as an enhancement of Bcl-2 expression in Gynostemma pentaphyllum-treated groups compared to the control. In addition, Gynostemma pentaphyllum remarkably improved pathologic changes of testicular tissue, reduced the content of malondialdehyde (MDA), and increased the activity of superoxide dismutase (SOD) caused by Zearalenone. Taken together, these results suggest that Gynostemma pentaphyllum protects against toxicity caused by Zearalenone through anti-oxidation and anti-apoptosis via the regulation of Bax and Bcl-2 expression.
本研究旨在探讨绞股蓝对玉米赤霉烯酮诱导的雄性小鼠生殖细胞凋亡的影响。50 只 25 日龄昆明雄性小鼠随机分为 5 组：A 组为空白对照（10%乙醇，0.5ml/天）；B 组给予 10μg 玉米赤霉烯酮/天；C、D、E 组分别给予 10μg 玉米赤霉烯酮和 50、100、200mg/kg 绞股蓝。结果表明，与对照组相比，绞股蓝能显著降低玉米赤霉烯酮诱导的雄性生殖细胞凋亡率，下调 Bax 表达，上调 Bcl-2 表达，对雄性生殖细胞具有明显的保护作用。此外，绞股蓝还能明显改善睾丸组织的病理变化，降低玉米赤霉烯酮所致的丙二醛（MDA）含量升高和超氧化物歧化酶（SOD）活性降低。综上，绞股蓝通过调节 Bax 和 Bcl-2 表达，发挥抗氧化和抗凋亡作用，从而减轻玉米赤霉烯酮的毒性。
