Laboratório de Virologia Aplicada, Departamento de Microbiologia e Parasitologia, Universidade Federal de Santa Catarina, Florianópolis, Santa Catarina State, Brazil.
Water Sci Technol. 2010;61(2):537-44. doi: 10.2166/wst.2010.845.
Sewage sludge and treated wastewater when contaminated with enteric virus and discharged into the environment, could pose a human health risk. The aim of study was to verify the presence and viability of enteric viruses in sewage sludge and treated wastewater at a local sewage plant in Florianopolis city, Brazil. Sewage sludge was concentrated by organic flocculation and polyethylene glycol precipitation and wastewater by electronegative membrane filtration and ultrafiltration by Centriprep Concentrator. Adenovirus (AdV), hepatitis A virus (HAV), and Rotavirus (RV) were examined for all samples for 12 months and Poliovirus (PV) was also tested for in sewage sludge samples. AdV was the most prevalent in both kind of samples, followed by RV, PV (in sludge) and HAV. Viral viability by cell culture (ICC-PCR) was: AdV: 100%, HAV: 16.7%, PV: 91.7%, RV: 25% in sludge and AdV: 66.6%, HAV: 66.6% and RV: 0% in wastewater. IFA for AdV in sludge ranged from 70 to 300 FFU/ml. QPCR for AdV ranged from 4.6 x 10(4) to 1.2 x 10(6) and from 50 to 1.3 x 10(4) gc/ml in sludge and wastewater, respectively. HAV quantification in sludge ranged from 3.1 x 10(2) to 5.4 x 10(2) gc/ml. In conclusion, it was possible to correlate presence and viability of enteric viruses in the environmental samples analyzed.
污水污泥和经处理的废水如果受到肠道病毒污染并排放到环境中，可能会对人类健康构成威胁。本研究的目的是验证巴西弗洛里亚诺波利斯市当地污水处理厂的污水污泥和处理废水中肠道病毒的存在和存活情况。通过有机絮凝和聚乙二醇沉淀浓缩污泥，通过电负性膜过滤和 Centriprep 浓缩器超滤浓缩废水。对所有样品进行了 12 个月的腺病毒 (AdV)、甲型肝炎病毒 (HAV) 和轮状病毒 (RV) 检测，并对污泥样品进行了脊髓灰质炎病毒 (PV) 检测。两种类型的样品中，AdV 最为普遍，其次是 RV、PV（在污泥中）和 HAV。细胞培养（ICC-PCR）的病毒活力为：AdV：100%，HAV：16.7%，PV：91.7%，RV：25%在污泥中，AdV：66.6%，HAV：66.6%和 RV：0%在废水中。IFA 法在污泥中的 AdV 范围为 70 至 300 FFU/ml。qPCR 法在污泥中的 AdV 范围为 4.6 x 10(4) 至 1.2 x 10(6)，在污泥和废水中分别为 50 至 1.3 x 10(4)gc/ml。污泥中 HAV 的定量范围为 3.1 x 10(2) 至 5.4 x 10(2)gc/ml。综上所述，本研究能够在分析的环境样本中检测到肠道病毒的存在和活力。
