采用分子方法对两种从污水污泥中回收肠病毒的提取方法进行比较研究。
Comparative study of two extraction methods for enteric virus recovery from sewage sludge by molecular methods.
机构信息
Departamentode Microbiologia e Parasitologia, Laboratório de Virologia Aplicada, Universidade Federal de Santa Catarina, Florianópolis, SC 88040-900, Brasil.
出版信息
Mem Inst Oswaldo Cruz. 2009 Jul;104(4):576-9. doi: 10.1590/s0074-02762009000400007.
The aim of this study was to compare two nucleic acid extraction methods for the recovery of enteric viruses from activated sludge. Test samples were inoculated with human adenovirus (AdV), hepatitis A virus (HAV), poliovirus (PV) and rotavirus (RV) and were then processed by an adsorption-elution-precipitation method. Two extraction methods were used: an organic solvent-based method and a silica method. The organic-based method was able to recoup 20% of the AdV, 90% of the RV and 100% of both the PV and HAV from seeded samples. The silica method was able to recoup 1.8% of the AdV and 90% of the RV. These results indicate that the organic-based method is more suitable for detecting viruses in sewage sludge.
本研究旨在比较两种从活性污泥中回收肠病毒的核酸提取方法。测试样本接种了人腺病毒（AdV）、甲型肝炎病毒（HAV）、脊髓灰质炎病毒（PV）和轮状病毒（RV），然后通过吸附-洗脱-沉淀法进行处理。使用了两种提取方法：一种基于有机溶剂的方法和一种硅胶方法。基于有机的方法能够从接种样本中回收 20%的 AdV、90%的 RV 以及 100%的 PV 和 HAV。硅胶法能够回收 1.8%的 AdV 和 90%的 RV。这些结果表明，基于有机的方法更适合检测污水污泥中的病毒。
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