Department of Plant Pathology, College of Plant Protection, Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects, Ministry of Agriculture, Nanjing Agricultural University, 210095, Nanjing, People's Republic of China.
Transgenic Res. 2010 Oct;19(5):841-7. doi: 10.1007/s11248-010-9365-x. Epub 2010 Jan 27.
hrf2 gene is a member of the harpin-encoding gene family of rice-pathogenic bacterium Xanthomonas oryzae pv. oryzicola. In our previous studies, we observed that harpin(Xooc) could elicit hypersensitive cell death in non-host plants, induce disease and insect resistance in plants, and enhance plant growth. In this study, the rapeseed cultivar, Yangyou 4, was genetically engineered via Agrobacterium-mediated transformation to express the hrf2 gene. Polymerase chain reaction (PCR) and southern blot analyses of T(1) generation of transgenic rapeseed revealed stable integration and expression of the inserted gene hrf2. In addition, the resistance to Sclerotinia sclerotiorum was greatly enhanced. A comparison between agronomic characters of transgenic and control lines displayed significant differences in terms of plant height, stem width, number of pods per plant, number of seeds per pod, 1,000-seed weight, and seed yield per plant. Among lines with resistance to S. sclerotiorum, T(1)1 had improved agronomic traits compared with controls with a 22.7% seed yield increase. These results suggest that the introduction of the hrf2 gene into rapeseed can be an effective strategy for enhancing resistance to S. sclerotiorum.
hrf2 基因是水稻致病菌黄单胞菌 pv.oryzicola 中 harpin 编码基因家族的一个成员。在我们之前的研究中，我们观察到 harpin(Xooc)可以在非宿主植物中引发过敏细胞死亡，诱导植物的疾病和昆虫抗性，并促进植物生长。在这项研究中，油菜品种扬油 4 通过农杆菌介导的转化进行了基因工程改造，以表达 hrf2 基因。T(1)代转基因油菜的聚合酶链反应(PCR)和Southern 印迹分析显示插入基因 hrf2 的稳定整合和表达。此外，对核盘菌的抗性大大增强。转基因和对照系农艺性状的比较显示，在株高、茎宽、每株荚数、每荚种子数、千粒重和单株产量方面存在显著差异。在对 S. sclerotiorum 具有抗性的系中，T(1)1 与对照相比具有改善的农艺性状，种子产量增加了 22.7%。这些结果表明，将 hrf2 基因导入油菜可以成为增强对核盘菌抗性的有效策略。
