Cambridge Laboratory, AFRC Institute of Plant Science Research, John Innes Centre, Colney Lane, NR4 7UJ, Norwich, UK.
Theor Appl Genet. 1992 Aug;84(5-6):585-91. doi: 10.1007/BF00224156.
The objective of this study was to separate and determine effects on the field performance of transgenic potatoes that originate from the tissue culture process of transformation and from the genes inserted. The constructs introduced contained the reporter gene for betaglucuronidase (GUS) under the control of the patatin promoter (four different constructs) and the neomycin phosphotransferase gene under the control of the nopaline synthase promoter. Both genes might be expected to have a neutral effect on plant phenotype. The field performance of transgenic plants (70 independent transformants) was compared with non-transgenic plants regenerated from tuber discs by adventitious shoot formation and from shoot cultures established from tuber nodal cuttings. Plants from all three treatments were grown in a field trial from previously field-grown tubers, and plant performance was measured in terms of plant height at flowering, weight of tubers, number of tubers, weight of large tubers and number of large tubers. There was evidence of somaclonal variation among the transgenic plants; mean values for all characters were significantly lower and variances generally higher than from plants derived from nodal shoot cultures. A similar change in means and variances was observed for the non-transgenic tuber-disc regenerants when compared with shoot culture plants. Plant height, tuber weight and tuber number were, however, significantly lower in transgenic plants than in tuber-disc regenerants, suggesting an effect on plant performance either of the tissue culture process used for transformation or of the genes inserted. There were significant differences between constructs for all five plant characters. The construct with the smallest segment of patatin promoter and the lowest level of tuber specificity for GUS expression had the lowest values for all five characters. It is proposed that the nature of GUS expression is influencing plant performance. There was no indication that the NPTII gene, used widely in plant transformation, has any substantial effect on plant performance in the field.
本研究的目的是分离和确定源自转化组织培养过程和插入基因的转基因马铃薯田间表现的影响。引入的构建体包含受 patatin 启动子（四个不同的构建体）控制的β-葡萄糖醛酸酶（GUS）报告基因和受胭脂碱合成酶启动子控制的新霉素磷酸转移酶基因。这两个基因都有可能对植物表型产生中性影响。通过不定芽形成从块茎外植体再生的和从块茎节间切段建立的茎培养物再生的非转基因植物（70 个独立转化体）与转基因植物的田间表现进行了比较。所有三种处理的植物都在先前田间生长的块茎的田间试验中生长，并根据开花时的植物高度、块茎重量、块茎数量、大薯重量和大薯数量来衡量植物性能。转基因植物存在体细胞变异的证据；所有性状的平均值明显较低，方差普遍高于来自节间芽培养的植物。与茎培养植物相比，非转基因块茎外植体再生植物的平均值和方差也发生了类似的变化。然而，与块茎外植体再生植物相比，转基因植物的株高、块茎重量和块茎数量显著降低，这表明转化中使用的组织培养过程或插入的基因对植物性能有影响。所有五个植物性状的构建体之间都存在显著差异。具有 patatin 启动子最小片段和 GUS 表达最低块茎特异性的构建体的所有五个性状的值均最低。据推测，GUS 表达的性质正在影响植物性能。没有迹象表明，广泛用于植物转化的 NPTII 基因在田间对植物性能有任何实质性影响。
