Department of Biochemistry, University of Kerala, Kariavattom, Thiruvananthapuram, India.
Biol Trace Elem Res. 2010 Dec;138(1-3):202-11. doi: 10.1007/s12011-009-8597-1. Epub 2010 Jan 27.
NFκB is a major transcription factor that controls the expression of various genes. Its activation is a complex process that can be triggered by many agents and one among them is reactive oxygen species. The aim of this study was to investigate the effect of selenium on NFκB activation in rats induced with myocardial infarction by isoproterenol (ISP). The markers of myocardial infarction showed increased activity in the serum of rats induced with ISP compared to the group that was pretreated with selenium along with ISP. Cellular selenium status was also found to be very low in the ISP-induced group of rats. The concentration of cytosolic NFκB was comparatively lower in the ISP group than in the group treated with selenium and ISP. Whereas higher levels of NFκB were found in the nuclear extract of the ISP-treated animals than in the selenium + ISP group. Elevated levels of malondialdehyde, hydroperoxides, and conjugated diens in the ISP-treated rats revealed the higher levels of oxidative stress in this group. Thus, our studies reveal the inhibitory effect of selenium in the nuclear translocation of NFκB during myocardial infarction. Histopathological studies of the heart also support the cardioprotective role of selenium.
NFκB 是一种主要的转录因子，可控制各种基因的表达。其激活是一个复杂的过程，可被许多因子触发，其中之一是活性氧。本研究旨在探讨硒对异丙肾上腺素（ISP）诱导心肌梗死大鼠 NFκB 激活的影响。与用硒预处理后再用 ISP 诱导的大鼠相比，ISP 诱导的大鼠血清中的心肌梗死标志物活性增加。还发现 ISP 诱导的大鼠的细胞硒状态非常低。与用硒和 ISP 处理的大鼠相比，ISP 组大鼠的细胞质 NFκB 浓度相对较低。而在 ISP 处理的动物的核提取物中发现 NFκB 水平较高，而在硒+ISP 组中则较低。ISP 处理的大鼠丙二醛、过氧化物和共轭二烯的水平升高，表明该组的氧化应激水平较高。因此，我们的研究揭示了硒在心肌梗死后 NFκB 核易位中的抑制作用。心脏的组织病理学研究也支持硒的心脏保护作用。
