Department of Central Research, The Third Clinical College, Jilin University, Xiantai Street No. 126, Changchun 130033, China.
Int J Vitam Nutr Res. 2009 Mar;79(2):104-16. doi: 10.1024/0300-9831.79.2.104.
There is a tendency for the incidence of diabetes in a population to increase with an improvement in living standards. This would imply the involvement of nutritional factors in the development of diabetes, and so nutritional considerations could be a key aspect in the research and development of an effective remedy for diabetes. In this study, combined micronutrients (selenium, vitamin E, vanadium, and chromium) were orally supplemented to streptozotocin-induced diabetic mice. Results showed that combined micronutrients could decrease the high blood glucose levels (p<0.05 or p<0.01) of diabetic mice. The protective effects of combined micronutrients on structures of beta-cells in pancreatic islets of diabetic mice were observed histopathologically and ultrastructurally. In addition, the supplementation of combined micronutrients increased insulin expression by beta-cells in pancreatic islets of diabetic mice at both translational and transcriptional levels. The immune molecular mechanisms involved were preliminarily regarded as downregulation of the expression of pathogenic T-helper 1 lymphocyte (Th1) cytokine tumor necrosis factor-alpha (TNF-alpha) (p<0.01) along with upregulation of the expression of protective T-helper 2 lymphocyte Th2 cytokine interleukin 10 (IL-10) (p<0.01) which ameliorates the Th1/Th2 imbalance in diabetes. In conclusion, supplementation of combined micronutrients to diabetic mice could effectively improve disordered glucose metabolism, protect islet structures, and improve the function of beta-cells in pancreatic islets, which are affected by differential regulation of the expression of Th1/Th2 cytokines involved in the pathogenesis of diabetes.
人群中糖尿病的发病率随着生活水平的提高而呈上升趋势。这意味着营养因素在糖尿病的发生发展中起一定作用，因此营养因素可能是研究和开发糖尿病有效治疗方法的关键方面。在这项研究中，联合给予微量元素（硒、维生素 E、钒和铬）经口补充给链脲佐菌素诱导的糖尿病小鼠。结果表明，联合给予微量元素可降低糖尿病小鼠的高血糖水平（p<0.05 或 p<0.01）。联合给予微量元素对糖尿病小鼠胰岛β细胞结构具有保护作用，这在组织病理学和超微结构上得到了观察。此外，联合给予微量元素可增加糖尿病小鼠胰岛β细胞的胰岛素表达，在翻译和转录水平上均如此。所涉及的免疫分子机制初步被认为是下调致病性辅助性 T 淋巴细胞（Th1）细胞因子肿瘤坏死因子-α（TNF-α）的表达（p<0.01），同时上调保护性辅助性 T 淋巴细胞 Th2 细胞因子白细胞介素 10（IL-10）的表达（p<0.01），这改善了糖尿病中 Th1/Th2 的失衡。总之，向糖尿病小鼠补充联合微量元素可有效改善葡萄糖代谢紊乱，保护胰岛结构，并改善受糖尿病发病机制中涉及的 Th1/Th2 细胞因子表达差异调节的胰岛β细胞功能。
