El-Borady Ola M, Othman Mohamed S, Atallah Heba H, Abdel Moneim Ahmed E
Institute for Nanoscience and Nanotechnology, Kafrelsheikh University, Kafrelsheikh, Egypt.
Faculty of Preparatory Year, University of Ha'il, Hail, KSA, Saudi Arabia.
Heliyon. 2020 May 30;6(5):e04045. doi: 10.1016/j.heliyon.2020.e04045. eCollection 2020 May.
This study was aimed to evaluate the efficacy of synthesized selenium nanoparticles (SeNPs) capped with glucose and polyvinyl-pyrrolidone (PVP) on the hyperglycemia and prooxidants/antioxidants imbalance present in model streptozotocin (STZ)-induced diabetic rats. SeNPs were synthesized and characterized. Twenty-four albino male rats were grouped into four different groups. After the rats were induced to have type 2 diabetes by STZ, the SeNPs-treated groups received a dose of 0.5 mg/ml of SeNPs for seven days. Plasma glucose and insulin levels, pancreatic insulin expression, the levels of lipid peroxidation (LPO), nitric oxide (NO), glutathione peroxidase (GPx) and glutathione (GSH) were evaluated. TEM images revealed the formation of semispherical particles with average size between 40 and 50 nm. SeNPs administration successfully reduced the hyperglycemia, raised the levels of insulin in both the pancreas and the plasma and restored the damaged pancreatic tissue. SeNPs also showed enhancement of the elimination of the diabetes-induced oxidative stress injuries by decreasing the pancreatic LPO and NO levels. Furthermore, the activities of the antioxidant enzyme GPx and GSH levels of the diabetic rats were increased. In conclusion, SeNPs capped with PVP could be used in the future as an agent that could manage Diabetes mellitus.
本研究旨在评估用葡萄糖和聚乙烯吡咯烷酮（PVP）包覆的合成硒纳米颗粒（SeNPs）对链脲佐菌素（STZ）诱导的糖尿病模型大鼠高血糖以及促氧化剂/抗氧化剂失衡的疗效。合成并表征了SeNPs。将24只白化雄性大鼠分为四组。大鼠经STZ诱导患2型糖尿病后，SeNPs处理组连续7天接受0.5mg/ml剂量的SeNPs。评估了血糖和胰岛素水平、胰腺胰岛素表达、脂质过氧化（LPO）、一氧化氮（NO）、谷胱甘肽过氧化物酶（GPx）和谷胱甘肽（GSH）水平。透射电镜图像显示形成了平均尺寸在40至50nm之间的半球形颗粒。给予SeNPs成功降低了高血糖，提高了胰腺和血浆中的胰岛素水平，并修复了受损的胰腺组织。SeNPs还通过降低胰腺LPO和NO水平，显示出增强了对糖尿病诱导的氧化应激损伤的消除作用。此外，糖尿病大鼠的抗氧化酶GPx活性和GSH水平增加。总之，PVP包覆的SeNPs未来可作为一种治疗糖尿病的药物。
