State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093, China.
J Environ Sci (China). 2009;21(11):1581-90. doi: 10.1016/s1001-0742(08)62459-6.
Interactions between bacteria and cyanobacteria have been suggested to have a potential to influence harmful algal bloom dynamics; however, little information on these interactions has been reported. In this study, the bacterial communities associated with five strains of Microcystis aeruginosa, three species of other Microcystis spp., and four representative species of non-Microcystis cyanobacteria were compared. Bacterial 16S rDNA fragments were amplified and separated by denaturing gradient gel electrophoresis (DGGE) followed by DNA sequence analysis. The similarities among bacterial communities associated with these cyanobacteria were compared to the digitized DGGE profiles using the cluster analyses. The bacterial community structure of all cyanobacterial cultures differed. Cluster analysis showed that the similarity values among M. aeruginosa cultures were higher than those of other cyanobacterial cultures. Sequence analysis of DGGE fragments indicated the presence of bacteria including, Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria, Bacteroidetes and Actinobacteria in the cyanobacterial cultures. Members of the Sphingomonadales were the prevalent group among the Microcystis-associated bacteria. The results provided further evidence for species-specific associations between cyanobacteria and heterotrophic bacteria, which are useful for understanding interactions between Microcystis and their associated bacteria.
细菌与蓝藻之间的相互作用被认为有可能影响有害藻类水华的动态；然而，关于这些相互作用的信息很少。在这项研究中，比较了与五种铜绿微囊藻、三种其他微囊藻属物种和四种代表性非微囊藻蓝藻菌株相关的细菌群落。通过变性梯度凝胶电泳（DGGE）扩增和分离细菌 16S rDNA 片段，然后进行 DNA 序列分析。使用聚类分析比较了与这些蓝藻相关的细菌群落与数字化 DGGE 图谱之间的相似性。所有蓝藻培养物的细菌群落结构都不同。聚类分析表明，铜绿微囊藻培养物之间的相似值高于其他蓝藻培养物之间的相似值。DGGE 片段的序列分析表明，蓝藻培养物中存在包括α变形菌、β变形菌、γ变形菌、拟杆菌门和放线菌门的细菌。鞘氨醇单胞菌目成员是微囊藻相关细菌中的主要群体。研究结果为蓝藻与异养细菌之间的种特异性关联提供了进一步的证据，这有助于理解微囊藻与其相关细菌之间的相互作用。
