Instituto de Biologia Experimental e Tecnológica, Avenida da República, Quinta do Marquês, Estação Agronómica Nacional, Oeiras, Portugal.
Int J Food Sci Nutr. 2010 Jun;61(4):357-68. doi: 10.3109/09637480903430990.
To date there are no licensed systemic or topical treatments in Europe or the USA for adenovirus infections. In the present paper, we evaluate the effect of a polyphenol-based grape extract (NE) obtained from Portuguese white-winemaking by-products, and Resveratrol in pure form, on adenovirus type 5 infection. For this purpose, recombinant adenovirus vectors (Ad-5) and a human-derived cell line (293) were used as models. The NE and Resveratrol at the used concentrations do not induce cell cytotoxicity or direct virucidal activity; however, they reduce 4.5 and 6.5 log (TCID(50)/ml) on total infectious Ad-5 production, respectively. The capacity of Ad-5 replication upon removal of NE and Resveratrol after 24 h post infection was also evaluated. In contrast to Resveratrol, the highest evaluated NE concentration inhibits irreversibly the Ad-5 replication. These results provide useful information for the use of NE and Resveratrol as potential sources of promising natural antiviral agents on Ad-5 infection.
迄今为止，在欧洲和美国还没有针对腺病毒感染的许可的系统或局部治疗方法。在本论文中，我们评估了一种多酚基葡萄提取物（来自葡萄牙白葡萄酒生产副产物的 NE）和白藜芦醇纯品对 5 型腺病毒感染的作用。为此，我们使用重组腺病毒载体（Ad-5）和人源性细胞系（293）作为模型。在使用的浓度下，NE 和白藜芦醇不会诱导细胞毒性或直接的病毒杀伤活性；然而，它们分别减少了 4.5 和 6.5 个对数（TCID50/ml）的总感染性 Ad-5 产量。在感染后 24 小时去除 NE 和白藜芦醇后，还评估了 Ad-5 复制的能力。与白藜芦醇相反，评估的最高 NE 浓度不可逆地抑制 Ad-5 复制。这些结果为使用 NE 和白藜芦醇作为对抗 Ad-5 感染的有前途的天然抗病毒药物的潜在来源提供了有用的信息。
