• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

透气乙烯袋用于小规模培养高致病性禽流感 H5N1 及其他病毒在鸡胚蛋。

Gas-permeable ethylene bags for the small scale cultivation of highly pathogenic avian influenza H5N1 and other viruses in embryonated chicken eggs.

机构信息

Energy and Life Sciences Division, Midwest Research Institute, 425 Volker Boulevard, Kansas City, Missouri, 64110, USA.

出版信息

Virol J. 2010 Jan 28;7:23. doi: 10.1186/1743-422X-7-23.

DOI:10.1186/1743-422X-7-23
PMID:20109234
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2825208/
Abstract

BACKGROUND

Embryonated chicken eggs (ECE) are sometimes used for the primary isolation or passage of influenza viruses, other viruses, and certain bacteria. For small-scale experiments with pathogens that must be studied in biosafety level three (BSL3) facilities, inoculated ECE are sometimes manipulated and maintained in small egg incubators within a biosafety cabinet (BSC). To simplify the clean up and decontamination of an egg incubator in case of egg breakage, we explored whether ethylene breather bags could be used to encase ECE inoculated with pathogens. This concept was tested by determining embryo survival and examining virus yields in bagged ECE.

RESULTS

Virus yields acceptable for many applications were attained when influenza-, alpha-, flavi-, canine distemper-, and mousepox viruses were propagated in ECE sealed within ethylene breather bags.

CONCLUSIONS

For many small-scale applications, ethylene breather bags can be used to encase ECE inoculated with various viruses.

摘要

背景

鸡胚（ECE）有时用于流感病毒、其他病毒和某些细菌的初次分离或传代。对于必须在三级生物安全设施（BSL3）中进行的小规模病原体实验，接种后的鸡胚有时在生物安全柜（BSC）内的小型孵育箱中进行操作和维持。为简化鸡蛋孵育箱在鸡蛋破裂时的清理和消毒工作，我们探讨了是否可以使用乙烯呼吸袋来包裹接种有病原体的鸡胚。通过确定囊内鸡胚的胚胎存活率和检测病毒产量，对该概念进行了测试。

结果

当流感病毒、甲型病毒、黄病毒、犬瘟热病毒和鼠痘病毒在乙烯呼吸袋内密封的鸡胚中增殖时，可获得适用于许多应用的可接受的病毒产量。

结论

对于许多小规模应用，乙烯呼吸袋可用于包裹接种有各种病毒的鸡胚。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c920/2825208/1a8df03da0f5/1743-422X-7-23-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c920/2825208/1a8df03da0f5/1743-422X-7-23-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c920/2825208/1a8df03da0f5/1743-422X-7-23-1.jpg

相似文献

1
Gas-permeable ethylene bags for the small scale cultivation of highly pathogenic avian influenza H5N1 and other viruses in embryonated chicken eggs.透气乙烯袋用于小规模培养高致病性禽流感 H5N1 及其他病毒在鸡胚蛋。
Virol J. 2010 Jan 28;7:23. doi: 10.1186/1743-422X-7-23.
2
Evaluation of different inactivation methods for high and low pathogenic avian influenza viruses in egg-fluids for antigen preparation.用于抗原制备的蛋液中高致病性和低致病性禽流感病毒不同灭活方法的评估。
J Virol Methods. 2015 Sep 15;222:28-33. doi: 10.1016/j.jviromet.2015.05.004. Epub 2015 May 18.
3
Assessing the susceptibility of highly pathogenic avian influenza H5N1 viruses to oseltamivir using embryonated chicken eggs.用鸡胚评估高致病性禽流感 H5N1 病毒对奥司他韦的敏感性。
Indian J Med Res. 2019 Nov;150(5):486-491. doi: 10.4103/ijmr.IJMR_845_18.
4
Evaluation and attempted optimization of avian embryos and cell culture methods for efficient isolation and propagation of low pathogenicity avian influenza viruses.评估并尝试优化用于高效分离和扩增低致病性禽流感病毒的禽胚胎及细胞培养方法。
Avian Dis. 2010 Mar;54(1 Suppl):622-6. doi: 10.1637/8837-040309-Reg.1.
5
Infection and propagation of Crimean-Congo hemorrhagic fever virus in embryonated chicken eggs.克里米亚-刚果出血热病毒在鸡胚中的感染和传播。
Virus Res. 2013 May;173(2):344-9. doi: 10.1016/j.virusres.2013.01.008. Epub 2013 Jan 23.
6
Avian influenza virus isolation, propagation, and titration in embryonated chicken eggs.禽流感病毒在鸡胚中的分离、增殖及滴定。
Methods Mol Biol. 2014;1161:125-40. doi: 10.1007/978-1-4939-0758-8_12.
7
Avian Influenza Virus Isolation, Propagation, and Titration in Embryonated Chicken Eggs.鸡胚中禽流感病毒的分离、繁殖和滴定。
Methods Mol Biol. 2020;2123:149-164. doi: 10.1007/978-1-0716-0346-8_12.
8
Egg fluids and cells of the chorioallantoic membrane of embryonated chicken eggs can select different variants of influenza A (H3N2) viruses.鸡胚卵黄囊液和绒毛尿囊膜细胞能够选择甲型流感病毒（H3N2）的不同变体。
Virology. 1995 Aug 1;211(1):302-6. doi: 10.1006/viro.1995.1405.
9
Biosafety Recommendations for Work with Influenza Viruses Containing a Hemagglutinin from the A/goose/Guangdong/1/96 Lineage.含有 A/goose/Guangdong/1/96 谱系血凝素的流感病毒工作的生物安全建议。
MMWR Recomm Rep. 2013 Jun 28;62(RR-06):1-7.
10
The inactivation of avian influenza virus subtype H5N1 isolated from chickens in Thailand by chemical and physical treatments.化学和物理处理对从泰国鸡中分离的 H5N1 亚型禽流感病毒的灭活作用。
Vet Microbiol. 2010 Jan 6;140(1-2):43-8. doi: 10.1016/j.vetmic.2009.07.008. Epub 2009 Jul 10.

引用本文的文献

1
Higher titers of some H5N1 and recent human H1N1 and H3N2 influenza viruses in Mv1 Lu vs. MDCK cells.Mv1 Lu 细胞中某些 H5N1 以及最近的人源 H1N1 和 H3N2 流感病毒的滴度更高。
Virol J. 2011 Feb 11;8:66. doi: 10.1186/1743-422X-8-66.
2
A nonlethal young domesticated ferret (Mustela putorius furo) model for studying pandemic influenza virus A/California/04/2009 (H1N1).一种用于研究甲型流感病毒A/加利福尼亚/04/2009（H1N1）大流行的非致死性幼年家养雪貂（白鼬）模型。
Comp Med. 2010 Oct;60(5):364-8.
3
Pandemic preparedness & the development of vaccines for H5N1 influenza at the Midwest Research Institute.

本文引用的文献

1
The Susceptibility of the Chorio-Allantoic Membrane of Chick Embryos to Infection with the Fowl-Pox Virus.鸡胚绒毛尿囊膜对禽痘病毒感染的易感性
Am J Pathol. 1931 May;7(3):209-222.5.
2
THE COURSE OF EXPERIMENTAL INFECTION OF THE CHICK EMBRYO WITH THE VIRUS OF EQUINE ENCEPHALOMYELITIS.实验性感染马传染性脑脊髓炎病毒的鸡胚过程。
J Exp Med. 1943 Apr 1;77(4):337-44. doi: 10.1084/jem.77.4.337.
3
Single amino acid substitutions in the hemagglutinin of influenza A/Singapore/21/04 (H3N2) increase virus growth in embryonated chicken eggs.
中西部研究所的大流行防范与H5N1流感疫苗研发
Mo Med. 2010 Sep-Oct;107(5):298-301.
4
Ferrets develop fatal influenza after inhaling small particle aerosols of highly pathogenic avian influenza virus A/Vietnam/1203/2004 (H5N1).雪貂吸入高致病性禽流感病毒 A/Vietnam/1203/2004（H5N1）的小颗粒气溶胶后会致命感染流感。
Virol J. 2010 Sep 15;7:231. doi: 10.1186/1743-422X-7-231.
5
Design, assembly, and validation of a nose-only inhalation exposure system for studies of aerosolized viable influenza H5N1 virus in ferrets.设计、组装和验证一种仅用于鼻腔吸入暴露的系统，用于研究雾化的具有感染力的 H5N1 流感病毒在雪貂中的作用。
Virol J. 2010 Jun 23;7:135. doi: 10.1186/1743-422X-7-135.
甲型流感病毒/新加坡/21/04（H3N2）血凝素中的单氨基酸替换增加了病毒在鸡胚中的生长。
Vaccine. 2006 Nov 10;24(44-46):6691-3. doi: 10.1016/j.vaccine.2006.05.062. Epub 2006 Jun 9.
4
Improvement of influenza A/Fujian/411/02 (H3N2) virus growth in embryonated chicken eggs by balancing the hemagglutinin and neuraminidase activities, using reverse genetics.通过反向遗传学平衡血凝素和神经氨酸酶活性来提高甲型流感病毒/福建/411/02（H3N2）在鸡胚中的生长。
J Virol. 2005 Jun;79(11):6763-71. doi: 10.1128/JVI.79.11.6763-6771.2005.
5
Detection and characterization of new influenza B virus variants in 2002.2002年新型乙型流感病毒变种的检测与特征分析
J Clin Microbiol. 2005 May;43(5):2345-9. doi: 10.1128/JCM.43.5.2345-2349.2005.
6
Genetically distant American Canine distemper virus lineages have recently caused epizootics with somewhat different characteristics in raccoons living around a large suburban zoo in the USA.基因上有差异的美洲犬瘟热病毒谱系最近在美国一个大型郊区动物园周边的浣熊中引发了具有不同特征的 epizootics。
Virol J. 2004 Sep 2;1:2. doi: 10.1186/1743-422X-1-2.
7
Effective primary isolation of wild-type canine distemper virus in MDCK, MV1 Lu and Vero cells without nucleotide sequence changes within the entire haemagglutinin protein gene and in subgenomic sections of the fusion and phospho protein genes.野生型犬瘟热病毒在MDCK、MV1 Lu和Vero细胞中能有效进行初次分离，且在整个血凝素蛋白基因以及融合蛋白和磷蛋白基因的亚基因组区域内无核苷酸序列变化。
J Virol Methods. 2004 Jun 15;118(2):147-57. doi: 10.1016/j.jviromet.2004.02.004.
8
Universal primer set for the full-length amplification of all influenza A viruses.用于甲型流感病毒全长扩增的通用引物组。
Arch Virol. 2001 Dec;146(12):2275-89. doi: 10.1007/s007050170002.
9
Enhanced recovery of avian influenza virus isolates by a combination of chicken embryo inoculation methods.通过鸡胚接种方法组合提高禽流感病毒分离株的回收率。
Avian Dis. 2001 Oct-Dec;45(4):1030-5.
10
Evolutionary relationships and systematics of the alphaviruses.甲病毒的进化关系与分类学
J Virol. 2001 Nov;75(21):10118-31. doi: 10.1128/JVI.75.21.10118-10131.2001.