Department of Otorhinolaryngology, Nippon Medical School, Tokyo, Japan.
Am J Rhinol Allergy. 2010 Jan-Feb;24(1):e14-8. doi: 10.2500/ajra.2010.24.3436.
Thymic stromal lymphopoietin (TSLP) is elevated in airway inflammatory diseases such as asthma and triggers dendritic cell-mediated activation of Th2 inflammatory responses. Although allergic chronic sinusitis is a Th2 inflammatory disease of the upper airway, the mechanism underlying the predominance of Th2 responses still has to be clarified. We investigated the expression of TSLP in cytokine-treated nasal polyp fibroblasts.
Fibroblast lines were established from nasal polyp tissues. Their expression of TSLP mRNA was evaluated by real-time reverse-transcription polymerase chain reaction. The amount of TSLP in the supernatants was measured by enzyme-linked immunosorbent assay.
Nasal polyp fibroblasts have the capacity to produce TSLP in response to stimulation by tumor necrosis factor (TNF) alpha. Combined stimulation with TNF-alpha + a Th2 cytokine (IL-4 or IL-13) was synergistic for TSLP production by the nasal polyp fibroblasts. This response was time and dose dependent. The TNF-alpha + Th2 cytokine (IL-4 or IL-13)-induced TSLP production was strongly inhibited by interferon gamma but not by IL-10.
These results suggest that nasal polyp fibroblasts play a role in the development and regulation of Th2-type inflammation in the upper airway by producing TSLP.
胸腺基质淋巴细胞生成素（TSLP）在气道炎症性疾病如哮喘中升高，并触发树突状细胞介导的 Th2 炎症反应的激活。虽然过敏性慢性鼻窦炎是上呼吸道的 Th2 炎症性疾病，但 Th2 反应优势的机制仍有待阐明。我们研究了细胞因子处理的鼻息肉成纤维细胞中 TSLP 的表达。
从鼻息肉组织中建立成纤维细胞系。通过实时逆转录聚合酶链反应评估 TSLP mRNA 的表达。通过酶联免疫吸附试验测量上清液中的 TSLP 量。
鼻息肉成纤维细胞具有在受到肿瘤坏死因子 (TNF) α刺激时产生 TSLP 的能力。TNF-α+Th2 细胞因子（IL-4 或 IL-13）的联合刺激对鼻息肉成纤维细胞的 TSLP 产生具有协同作用。这种反应是时间和剂量依赖性的。干扰素 γ强烈抑制 TNF-α+Th2 细胞因子（IL-4 或 IL-13）诱导的 TSLP 产生，但 IL-10 不能抑制。
这些结果表明，鼻息肉成纤维细胞通过产生 TSLP 在鼻气道的 Th2 型炎症的发展和调节中发挥作用。
