Life Science Division, Graduate School at Shenzhen, Tsinghua University, Shenzhen, PR China.
Fish Shellfish Immunol. 2010 Apr;28(4):695-700. doi: 10.1016/j.fsi.2010.01.011. Epub 2010 Jan 28.
TGF-beta is one of the pleiotropic cytokines and plays a pivotal role in immune regulation and orchestrating the subsequent healing response. Recombinant red seabream TGF-beta (rTGF-beta) mature peptide was expressed and purified under native conditions in vitro. Bio-assay showed that the rTGF-beta could significantly induce head kidney (HK) and peripheral blood (PB) leucocytes migration in a dose dependent manner, whereas the rTGF-beta suppressed HK and PB leucocyte migration when the leucocytes was activated by primed with lipopolysaccharide (LPS). Both enhancing and suppressing roles of rTGF-beta on the HKL and PBL chemotactic activity indicated that the fish TGF-beta shared the similar bipolar nature with mammalian TGF-beta. Furthermore, the results indicated that the activity of TGF-beta induction of leucocyte migration appears not to be an innate feature but function by regulation the chemokines activity. This is the first time we reported that fish TGF-beta has innate bipolar property in regulation of fish immune function.
TGF-β 是一种多功能细胞因子，在免疫调节和协调随后的愈合反应中发挥关键作用。重组红鲷 TGF-β（rTGF-β）成熟肽在体外的天然条件下表达和纯化。生物测定表明，rTGF-β 可以显著诱导头肾（HK）和外周血（PB）白细胞迁移，呈剂量依赖性，而当白细胞被脂多糖（LPS）预先激活时，rTGF-β 抑制 HK 和 PB 白细胞迁移。rTGF-β 对 HKL 和 PBL 趋化活性的增强和抑制作用表明，鱼类 TGF-β 与哺乳动物 TGF-β 具有相似的双极性性质。此外，结果表明，白细胞迁移的 TGF-β 诱导活性似乎不是先天特征，而是通过调节趋化因子活性起作用。这是我们首次报道鱼类 TGF-β 在调节鱼类免疫功能方面具有先天的双极性特性。
