GenoSplice technology, Centre Hayem, Hôpital Saint-Louis, 1 avenue Claude Vellefaux, 75010, Paris, France.
Nucleic Acids Res. 2010 May;38(9):2825-38. doi: 10.1093/nar/gkq008. Epub 2010 Jan 27.
It has been shown that alternative splicing is especially prevalent in brain and testis when compared to other tissues. To test whether there is a specific propensity of these tissues to generate splicing variants, we used a single source of high-density microarray data to perform both splicing factor and exon expression profiling across 11 normal human tissues. Paired comparisons between tissues and an original exon-based statistical group analysis demonstrated after extensive RT-PCR validation that the cerebellum, testis, and spleen had the largest proportion of differentially expressed alternative exons. Variations at the exon level correlated with a larger number of splicing factors being expressed at a high level in the cerebellum, testis and spleen than in other tissues. However, this splicing factor expression profile was similar to a more global gene expression pattern as a larger number of genes had a high expression level in the cerebellum, testis and spleen. In addition to providing a unique resource on expression profiling of alternative splicing variants and splicing factors across human tissues, this study demonstrates that the higher prevalence of alternative splicing in a subset of tissues originates from the larger number of genes, including splicing factors, being expressed than in other tissues.
已经表明，与其他组织相比，选择性剪接在大脑和睾丸中尤为普遍。为了测试这些组织是否具有产生剪接变体的特定倾向，我们使用单一来源的高密度微阵列数据，在 11 种正常人体组织中进行剪接因子和外显子表达谱分析。对组织之间的配对比较和基于原始外显子的统计组分析表明，经过广泛的 RT-PCR 验证，小脑、睾丸和脾脏具有最大比例的差异表达的选择性外显子。外显子水平的变化与更多的剪接因子在小脑、睾丸和脾脏中的高水平表达相关，而在其他组织中则较少。然而，这种剪接因子表达谱与更全面的基因表达模式相似，因为在小脑、睾丸和脾脏中，更多的基因具有高水平的表达。除了提供关于人类组织中选择性剪接变体和剪接因子表达谱的独特资源外，这项研究还表明，在某些组织中选择性剪接更为普遍的原因是更多的基因，包括剪接因子，在这些组织中表达的数量多于其他组织。
