Bhasi Ashwini, Philip Philge, Sreedharan Vipin T, Senapathy Periannan
Department of Human Genetics, Genome International Corp, 8000 Excelsior Drive, Madison, WI 53717, USA.
Genomics. 2009 Jul;94(1):48-54. doi: 10.1016/j.ygeno.2009.02.006. Epub 2009 Mar 11.
We have developed AspAlt-a web-based comparative analytical platform for exploring the variations in alternative transcription (AT) events and alternative splicing (AS) events in eukaryotes. AspAlt provides integrated access to 2.1 million AT-AS annotations from 1,58,876 multi-isoform genes and has the following user-friendly analytical features: (1) advanced graphical display to visualize and analyze AT-AS events in 46 eukaryotic genomes; (2) compare and identify the differences in AT-AS patterns among a group of genes specified by the user or among homologous gene groups; (3) inter-database comparative viewer to analyze the differences in the AT-AS annotations for the same gene among Ensembl, RefSeq and AceView databases; (4) dynamically classify and generate graphical plots of AT-AS events from mRNA annotations submitted by the user; and (5) download genomic AT-AS annotations of 46 eukaryotes in XML and tab-delimited formats. The AspAlt resource is available at http://66.170.16.154/AspAlt.
我们开发了AspAlt——一个基于网络的比较分析平台，用于探索真核生物中可变转录（AT）事件和可变剪接（AS）事件的变化。AspAlt提供了对来自158876个多异构体基因的210万个AT-AS注释的集成访问，并具有以下用户友好的分析功能：（1）先进的图形显示，以可视化和分析46个真核生物基因组中的AT-AS事件；（2）比较并识别用户指定的一组基因之间或同源基因组之间AT-AS模式的差异；（3）数据库间比较查看器，以分析Ensembl、RefSeq和AceView数据库中同一基因的AT-AS注释差异；（4）根据用户提交的mRNA注释动态分类并生成AT-AS事件的图形图；以及（5）以XML和制表符分隔格式下载46个真核生物的基因组AT-AS注释。可在http://66.170.16.154/AspAlt获取AspAlt资源。
