Division of Developmental Biology, Department of Cell and Molecular Biology, Tulane University, New Orleans, LA 70118, USA.
Development. 2010 Feb;137(4):551-9. doi: 10.1242/dev.042424.
The regenerating digit tip of mice is a novel epimorphic response in mammals that is similar to fingertip regeneration in humans. Both display restricted regenerative capabilities that are amputation-level dependent. Using this endogenous regeneration model in neonatal mice, we have found that noggin treatment inhibits regeneration, thus suggesting a bone morphogenetic protein (BMP) requirement. Using non-regenerating amputation wounds, we show that BMP7 or BMP2 can induce a regenerative response. BMP-induced regeneration involves the formation of a mammalian digit blastema. Unlike the endogenous regeneration response that involves redifferentiation by direct ossification (evolved regeneration), the BMP-induced response involves endochondral ossification (redevelopment). Our evidence suggests that BMP treatment triggers a reprogramming event that re-initiates digit tip development at the amputation wound. These studies demonstrate for the first time that the postnatal mammalian digit has latent regenerative capabilities that can be induced by growth factor treatment.
小鼠再生指尖是哺乳动物中一种新的表型反应，类似于人类指尖再生。两者都表现出与断肢水平相关的有限再生能力。在新生小鼠中使用这种内源性再生模型，我们发现 noggin 处理抑制了再生，因此表明需要骨形态发生蛋白 (BMP)。使用非再生性断肢伤口，我们表明 BMP7 或 BMP2 可以诱导再生反应。BMP 诱导的再生涉及哺乳动物指尖芽基的形成。与涉及直接成骨的内源性再生反应（进化再生）不同，BMP 诱导的反应涉及软骨内骨化（再发育）。我们的证据表明，BMP 处理触发了一个重新编程事件，该事件重新启动了断肢伤口处的指尖发育。这些研究首次表明，出生后哺乳动物的指尖具有潜在的再生能力，可以通过生长因子处理来诱导。
