Spatio-temporal patterns of ovarian development and gene silencing reduced fecundity in parthenogenetic .

The halophilic zooplankton brine shrimp has been used as an experimental animal in multidisciplinary studies. However, the reproductive patterns and its regulatory mechanisms in remain unclear. In this study, the ovarian development process of parthenogenetic () was divided into five stages, and oogenesis or egg formation was identified in six phases. The oogenesis mode was assumed to be polytrophic. We also traced the dynamic translocation of candidate germline stem cells (cGSCs) using EdU labelling and elucidated several key cytological events in oogenesis through haematoxylin and eosin staining and fluorescence imaging. Distinguished from the ovary structure of insects and crustaceans, germarium originated from ovariole buds and are located at the base of the ovarioles. RNA-seq based on five stages of ovarian development identified 2657 upregulated genes related to reproduction by pair-to-pair comparison. , , , , , and genes associated with cGSCs recognition and reproductive development were screened and verified using qPCR. Silencing of the gene in () at ovarian development Stage II led to a low level of gene expression (less than 10%) within 5 days, which resulted in variations in oogenesis-related gene expression and significantly inhibited vitellogenesis, impeded oocyte maturation, and eventually decreased the number of offspring. In conclusion, we have illustrated the patterns of ovarian development, outlined the key spatio-temporal features of oogenesis and identified the negative impacts of VgR gene knockdown on oogenesis using as an experimental animal. The findings of this study also lay a foundation for the further study of reproductive biology of invertebrates.