Pediatric Exercise Research Center, Department of Pediatrics, University Children's Hospital, University of California-Irvine, Orange, CA 92868, USA.
J Appl Physiol (1985). 2010 Jul;109(1):252-61. doi: 10.1152/japplphysiol.01291.2009. Epub 2010 Jan 28.
Exercise leads to a rapid change in the profile of gene expression in circulating neutrophils. MicroRNAs (miRNAs) have been discovered to play important roles in immune function and often act to attenuate or silence gene translation. We hypothesized that miRNA expression in circulating neutrophils would be affected by brief exercise. Eleven healthy men (19-30 yr old) performed 10, 2-min bouts of cycle ergometer exercise interspersed with 1-min rest at a constant work equivalent to approximately 76% of maximal oxygen uptake (Vo(2 max)). We used the Agilent Human miRNA V2 Microarray. A conservative statistical approach was used to determine that exercise significantly altered 38 miRNAs (20 had lower expression). Using RT-PCR, we verified the expression level changes from before to after exercise of seven miRNAs. In silico analysis showed that collectively 36 miRNAs potentially targeted 4,724 genes (2 of the miRNAs had no apparent gene targets). Moreover, when we compared the gene expression changes (n = 458) in neutrophils that have been altered by exercise, as previously reported, with the miRNAs altered by exercise, we identified three pathways, Ubiquitin-mediated proteolysis, Jak-STAT signaling pathway, and Hedgehog signaling pathway, in which an interaction of miRNA and gene expression was plausible. Each of these pathways is known to play a role in key mechanisms of inflammation. Brief exercise alters miRNA profile in circulating neutrophils in humans. These data support the hypothesis that exercise-associated changes in neutrophil miRNA expression play a role in neutrophil gene expression in response to physical activity.
运动导致循环中性粒细胞中基因表达谱的快速变化。已经发现 microRNAs（miRNAs）在免疫功能中发挥重要作用，并且通常起到减弱或沉默基因翻译的作用。我们假设循环中性粒细胞中的 miRNA 表达会受到短暂运动的影响。11 名健康男性（19-30 岁）以恒定的工作强度进行 10 次、2 分钟的自行车测力计运动，每次运动之间穿插 1 分钟的休息，工作强度约为最大摄氧量（Vo(2 max）的 76%。我们使用 Agilent Human miRNA V2 Microarray。采用保守的统计方法来确定运动显著改变了 38 个 miRNA（20 个 miRNA 的表达水平降低）。通过 RT-PCR，我们验证了运动前后七个 miRNA 的表达水平变化。计算机分析表明，36 个 miRNA 可能共同靶向 4724 个基因（其中 2 个 miRNA 没有明显的基因靶标）。此外，当我们将之前报道的运动改变中性粒细胞中的基因表达变化（n = 458）与运动改变的 miRNA 进行比较时，我们确定了三个途径，泛素介导的蛋白水解、Jak-STAT 信号通路和 Hedgehog 信号通路，其中 miRNA 和基因表达的相互作用是合理的。这些途径中的每一个都已知在炎症的关键机制中发挥作用。短暂运动改变了人类循环中性粒细胞中的 miRNA 谱。这些数据支持这样的假设，即运动相关的中性粒细胞 miRNA 表达变化在对身体活动的中性粒细胞基因表达中发挥作用。
