人体运动诱导的循环祖细胞动员依赖于一氧化氮，而南亚男性的这种动员能力减弱。

Human exercise-induced circulating progenitor cell mobilization is nitric oxide-dependent and is blunted in South Asian men.

机构信息

Division of Cardiovascular and Diabetes Research, Leeds Multidisciplinary Cardiovascular Research Centre, LIGHT Laboratories, Clarendon Way, The University of Leeds, Leeds, LS2 9JT, United Kingdom.

出版信息

Arterioscler Thromb Vasc Biol. 2010 Apr;30(4):878-84. doi: 10.1161/ATVBAHA.109.201012. Epub 2010 Jan 28.

DOI:10.1161/ATVBAHA.109.201012

PMID:20110574

Abstract

OBJECTIVE

Circulating progenitor cells (CPC) have emerged as potential mediators of vascular repair. In experimental models, CPC mobilization is critically dependent on nitric oxide (NO). South Asian ethnicity is associated with reduced CPC. We assessed CPC mobilization in response to exercise in Asian men and examined the role of NO in CPC mobilization per se.

METHODS AND RESULTS

In 15 healthy, white European men and 15 matched South Asian men, CPC mobilization was assessed during moderate-intensity exercise. Brachial artery flow-mediated vasodilatation was used to assess NO bioavailability. To determine the role of NO in CPC mobilization, identical exercise studies were performed during intravenous separate infusions of saline, the NO synthase inhibitor L-NMMA, and norepinephrine. Flow-mediated vasodilatation (5.8%+/-0.4% vs 7.9%+/-0.5%; P=0.002) and CPC mobilization (CD34(+)/KDR(+) 53.2% vs 85.4%; P=0.001; CD133(+)/CD34(+)/KDR(+) 48.4% vs 73.9%; P=0.05; and CD34(+)/CD45(-) 49.3% vs 78.4; P=0.006) was blunted in the South Asian group. CPC mobilization correlated with flow-mediated vasodilatation and l-NMMA significantly reduced exercise-induced CPC mobilization (CD34(+)/KDR(+) -3.3% vs 68.4%; CD133(+)/CD34(+)/KDR(+) 0.7% vs 71.4%; and CD34(+)/CD45(-) -30.5% vs 77.8%; all P<0.001).

CONCLUSIONS

In humans, NO is critical for CPC mobilization in response to exercise. Reduced NO bioavailability may contribute to imbalance between vascular damage and repair mechanisms in South Asian men.

摘要

目的

循环祖细胞（CPC）已成为血管修复的潜在介质。在实验模型中，CPC 的动员严重依赖于一氧化氮（NO）。南亚种族与 CPC 减少有关。我们评估了亚洲男性对运动的反应中 CPC 的动员，并研究了 NO 本身在 CPC 动员中的作用。

方法和结果

在 15 名健康的白种欧洲男性和 15 名匹配的南亚男性中，在中等强度运动期间评估了 CPC 的动员。肱动脉血流介导的血管舒张用于评估 NO 生物利用度。为了确定 NO 在 CPC 动员中的作用，在静脉内单独输注生理盐水、一氧化氮合酶抑制剂 L-NMMA 和去甲肾上腺素期间进行了相同的运动研究。血流介导的血管舒张（5.8%+/-0.4%对 7.9%+/-0.5%；P=0.002）和 CPC 动员（CD34(+)/KDR(+) 53.2%对 85.4%；P=0.001；CD133(+)/CD34(+)/KDR(+) 48.4%对 73.9%；P=0.05；和 CD34(+)/CD45(-) 49.3%对 78.4；P=0.006）在南亚组中减弱。CPC 动员与血流介导的血管舒张相关，L-NMMA 显著降低运动诱导的 CPC 动员（CD34(+)/KDR(+) -3.3%对 68.4%；CD133(+)/CD34(+)/KDR(+) 0.7%对 71.4%；和 CD34(+)/CD45(-) -30.5%对 77.8%；所有 P<0.001）。