Department of Food and Nutrition, Hanyang University, Seoul, South Korea.
Ann Nutr Metab. 2010;56(2):106-12. doi: 10.1159/000277663. Epub 2010 Jan 28.
BACKGROUND/AIMS: Soy isoflavones have been reported to prevent bone loss in rat models of osteoporosis. However, the effect of the natural soybeans that people consume in their diet needs to be explored. In the present study, we evaluated the effects of yellow and black soybeans (Glycine max), and sword beans (Canavalia gladiate) on bone mineral density (BMD), bone mineral content (BMC), and bone biomarkers in ovariectomized (OVX) rats.
Seven-week-old female Sprague-Dawley rats were raised for 2 weeks on a calcium-free diet based on the American Institute of Nutrition (AIN)-93M diets. All the rats received OVX, were randomized to one of four groups, and given one of the diets supplemented with casein, yellow soybean, black soybean, and sword bean for 10 weeks. BMD, BMC, osteocalcin (OC; a bone formation biomarker), deoxypyridinoline (DPD; a bone resorption biomarker), and TNF-alpha (a bone resorption cytokine) were assessed.
In the femur and lumbar spine, BMD and BMC were significantly higher in the various bean groups than in the casein group. Among the bean groups, femur and spine BMD were significantly higher in the yellow soybean and sword bean groups than in the black soybean group. Femur BMC was the highest in the yellow soybean group, and spine BMC was not significantly different between the various bean groups. Plasma OC concentrations and urinary DPD excretion were significantly higher in the casein group than in the various bean groups. The sword bean group showed significantly lower OC and DPD levels than the yellow and black soybean groups. There were no significant differences between the yellow and black soybean groups. TNF-alpha concentrations were not significantly different between the four groups.
Consumption of yellow and black soybeans, and sword beans had a definite protective effect on bone loss in OVX rats by inhibiting bone turnover and preventing bone resorption. Furthermore, consumption of sword beans may help prevent postmenopausal osteoporosis.
背景/目的：大豆异黄酮已被报道可预防骨质疏松症大鼠模型中的骨丢失。然而，人们在饮食中所食用的天然大豆的效果仍需探索。在本研究中，我们评估了黄大豆和黑大豆（Glycine max）以及刀豆（Canavalia gladiate）对去卵巢（OVX）大鼠的骨密度（BMD）、骨矿物质含量（BMC）和骨生物标志物的影响。
7 周龄雌性 Sprague-Dawley 大鼠在基于美国营养学会（AIN）-93M 饮食的无钙饮食上饲养 2 周。所有大鼠均接受 OVX，并随机分为 4 组中的一组，并给予补充酪蛋白、黄大豆、黑大豆和刀豆的饮食 10 周。评估骨密度、骨矿物质含量、骨钙素（OC；骨形成生物标志物）、脱氧吡啶啉（DPD；骨吸收生物标志物）和 TNF-α（骨吸收细胞因子）。
在股骨和腰椎，各豆类组的 BMD 和 BMC 均显著高于酪蛋白组。在豆类组中，黄大豆和刀豆组的股骨和腰椎 BMD 均显著高于黑大豆组。股骨 BMC 最高的是黄大豆组，而各豆类组之间的腰椎 BMC 无显著差异。血浆 OC 浓度和尿 DPD 排泄量在酪蛋白组中显著高于各豆类组。刀豆组的 OC 和 DPD 水平显著低于黄大豆和黑大豆组。黄大豆和黑大豆组之间无显著差异。TNF-α浓度在四组间无显著差异。
食用黄大豆、黑大豆和刀豆对 OVX 大鼠的骨丢失具有明确的保护作用，可通过抑制骨转换和防止骨吸收。此外，食用刀豆可能有助于预防绝经后骨质疏松症。
