Department of Orthopeadics, Xijing Hospital, The Fourth Military Medical University, No 17 Changlexi Road, Xi'an 710032, China.
J Ethnopharmacol. 2011 Oct 11;137(3):1083-8. doi: 10.1016/j.jep.2011.07.017. Epub 2011 Jul 18.
Recent research has confirmed that Cibotium barometz could inhibits osteoclast formation with no affect on BMM cell viability. However, the influence of Cibotium barometz on osteoporosis in animals is relatively unknown. The purpose of this study is to systemically investigate the effects of Cibotium barometz extract (CBE) on ovariectomy-induced osteoporosis in rats.
A total of Seventy-two 3-month-old female Sprague-Dawley rats were used and randomly divided into sham-operated group and five ovariectomized (OVX) groups: OVX with vehicle; OVX with 17β-estradiol (E2, 25 μg/kg/day); OVX with CBE of graded doses (100, 300, or 500 mg/kg/day). Daily oral administration of E2 or CBE began 4 weeks after the surgery and lasted for 16 weeks. Bone mass, bone turnover and strength were analyzed by DEXA, biochemical markers and three-point bending test. The trabecular bone microarchitecture was evaluated by MicroCT.
CBE prevented total BMD decrease in the femur induced by OVX, which was accompanied by a significant decrease in skeletal remodeling, as was evidenced by the decreased levels of the bone turnover markers, such as osteocalcin (OC), alkaline phosphatese (ALP), deoxypyridinoline (DPD), and urinary Ca and P excretions. The treatment could also enhance the bone strength and prevent the deterioration of trabecular microarchitecture.
The present study indicated that Cibotium barometz extract might be a potential alternative medicine for the prevention and treatment of postmenopausal osteoporosis.
最近的研究证实，翠云草可以抑制破骨细胞的形成，而对 BMM 细胞活力没有影响。然而，翠云草对动物骨质疏松症的影响尚不清楚。本研究旨在系统研究翠云草提取物（CBE）对去卵巢大鼠骨质疏松症的影响。
共使用 72 只 3 月龄雌性 Sprague-Dawley 大鼠，随机分为假手术组和 5 个去卵巢组：去卵巢+载体；去卵巢+17β-雌二醇（E2，25μg/kg/天）；去卵巢+CBE 分级剂量（100、300 或 500mg/kg/天）。手术后 4 周开始每天口服 E2 或 CBE，持续 16 周。通过 DEXA、生化标志物和三点弯曲试验分析骨量、骨转换和骨强度。通过 MicroCT 评估小梁骨微结构。
CBE 可预防去卵巢引起的股骨总骨密度降低，同时伴有骨骼重塑明显减少，骨转换标志物如骨钙素（OC）、碱性磷酸酶（ALP）、脱氧吡啶啉（DPD）和尿钙、磷排泄水平降低。该治疗还可以增强骨强度，防止小梁微结构恶化。
本研究表明，翠云草提取物可能是预防和治疗绝经后骨质疏松症的一种潜在替代药物。
