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Enzyme linked immunosorbent assay (ELISA) for human IgG (Gm) allotype determination.

作者信息

Ota T, Higashi S, Suzuki H, Eto S

机构信息

First Department of Internal Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan.

出版信息

Mol Immunol. 1991 Jan-Feb;28(1-2):51-6. doi: 10.1016/0161-5890(91)90086-y.

Abstract

An inhibition enzyme-linked immunosorbent assay (inhibition-ELISA) was developed for the quantitative determination of human IgG (Gm) allotypes using rabbit anti-Gm antisera, alkaline-phosphatase-conjugated goat anti-rabbit IgG and, as the calibrant, purified human myeloma proteins possessing the relevant Gm allotype. The assay is reproducible and can detect as little as 10 ng/ml of G1m(a), G2m(n) or G3m(st), and 100 ng/ml of G1m(f) or G3m(g). Using this assay, the "gene dosage effect" and "allelic balance" in healthy Japanese were studied.

摘要

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