Bird P, Lowe J, Jefferis R, de Lange G, van Loghem E, Ling N R
Vox Sang. 1984;47(5):366-72. doi: 10.1111/j.1423-0410.1984.tb04141.x.
The properties of a group of mouse monoclonal antibodies (McAbs) specific for the human G1m(f) allotype marker on immunoglobulin G are described. The specificity of all 5 McAbs was anti-Gm(f) in haemagglutination assays detecting the G1m(f) determinant on 6 ng of purified G1m(f) paraproteins. A high dilution (greater than or equal to 1/10(4)) could be used for the majority of McAbs in this assay. In Elisa the G1m(f) marker could be detected in homozygote G1m(f+z-) sera at a serum dilution of greater than or equal to 1/10(4). In Elisa assays the G1m(f) specificity was lost when IgG was bound directly to polystyrene but was restored when IgG was bound via anti-human IgG to the polystyrene plate. A possible conformational change in IgG to account for this loss of specificity is discussed. It is expected that these McAbs with their high titre and increased sensitivity over conventional Gm antisera will allow more detailed analysis of the Gm marker system.
本文描述了一组针对免疫球蛋白G上人类G1m(f)同种异型标记的小鼠单克隆抗体（McAbs）的特性。在血凝试验中，所有5种McAbs对6 ng纯化的G1m(f)副蛋白上的G1m(f)决定簇均具有抗Gm(f)特异性。在该试验中，大多数McAbs可使用高稀释度（大于或等于1/10(4)）。在酶联免疫吸附测定（ELISA）中，在血清稀释度大于或等于1/10(4)时，可在纯合子G1m(f+z-)血清中检测到G1m(f)标记。在ELISA试验中，当IgG直接结合到聚苯乙烯上时，G1m(f)特异性丧失，但当IgG通过抗人IgG结合到聚苯乙烯板上时，特异性得以恢复。文中讨论了IgG可能发生的构象变化以解释这种特异性丧失的现象。预计这些具有高滴度且比传统Gm抗血清灵敏度更高的McAbs将有助于对Gm标记系统进行更详细的分析。
