对具有针对人IgG同种异型假定特异性的单克隆抗体的评估。

Evaluation of monoclonal antibodies with putative specificity for human IgG allotypes.

作者信息

Nelson P N, Fletcher S M, de Lange G G, van Leeuwen A M, Goodall M, Jefferis R

机构信息

Department of Immunology, Medical School, University of Birmingham, UK.

出版信息

Vox Sang. 1990;59(3):190-7. doi: 10.1111/j.1423-0410.1990.tb00858.x.

DOI:10.1111/j.1423-0410.1990.tb00858.x

PMID:2264322

Abstract

Fourteen monoclonal antibodies (MAbs) of putative specificity for human IgG allotypes and isoallotypes were evaluated for reactivity and specificity in 8 different assay systems. The study showed that the MAbs tested could be classified into 1 of 4 groups: those exhibiting allotypic specificity regardless of the assay system, allotypic specificity dependent on the assay system, isoallotypic specificity, and those showing neither allotypic nor isoallotypic specificity. These observations were presumably dependent on antigen presentation, epitope integrity and/or antibody multispecificity. For the G1m(a), G1m(f), G1m(z), G3m(g) and G3m(u) specificities, MAbs have been produced which can be used for routine typing purposes in defined haemagglutination and enzyme-linked immunosorbent assay systems. MAbs are also available that show 'non-g' isoallotypic specificity.

摘要

对14种针对人IgG同种异型和异同种异型具有假定特异性的单克隆抗体（MAb）在8种不同检测系统中进行了反应性和特异性评估。研究表明，所测试的MAb可分为4组中的1组：无论检测系统如何均表现出同种异型特异性的MAb、依赖于检测系统的同种异型特异性的MAb、异同种异型特异性的MAb以及既不表现出同种异型也不表现出异同种异型特异性的MAb。这些观察结果可能取决于抗原呈递、表位完整性和/或抗体多特异性。对于G1m(a)、G1m(f)、G1m(z)、G3m(g)和G3m(u)特异性，已产生了可用于特定血凝和酶联免疫吸附检测系统中常规分型目的的MAb。也有显示“非g”异同种异型特异性的MAb。