Zimowska M, Duchesnay A, Dragun P, Oberbek A, Moraczewski J, Martelly I
Department of Cytology, Institute of Zoology, Faculty of Biology, University of Warsaw, Warsaw, Poland.
Int J Cell Biol. 2009;2009:659372. doi: 10.1155/2009/659372. Epub 2009 May 10.
When injured by crushing, the repair of the slow-twitch soleus rat muscle, unlike the fast-twitch EDL, is associated with fibrosis. As TGFbeta1, whose activity can be controlled by glycosaminoglycans (GAG), plays a major role in fibrosis, we hypothesized that levels of TGFbeta1 and GAG contents could account for this differential quality of regeneration. Here we show that the regeneration of the soleus was accompanied by elevated and more sustained TGFbeta1 level than in the EDL. Neutralization of TGFbeta1 effects by antibodies to TGFbeta1 or its receptor TGFbeta-R1 improved muscle repair, especially of the soleus muscle, increased in vitro growth of myoblasts, and accelerated their differentiation. These processes were accompanied by alterations of GAG contents. These results indicate that the control of TGFbeta1 activity is important to improve regeneration of injured muscle and accelerate myoblast differentiation, in part through changes in GAG composition of muscle cell environment.
当受到挤压损伤时，与快肌型趾长伸肌（EDL）不同，慢肌型比目鱼肌的修复与纤维化有关。由于转化生长因子β1（TGFbeta1）的活性可由糖胺聚糖（GAG）控制，且在纤维化过程中起主要作用，我们推测TGFbeta1水平和GAG含量可解释这种再生质量的差异。在此我们表明，比目鱼肌的再生伴随着比EDL中更高且更持久的TGFbeta1水平。用抗TGFbeta1或其受体TGFbeta - R1的抗体中和TGFbeta1的作用可改善肌肉修复，尤其是比目鱼肌的修复，增加成肌细胞的体外生长，并加速其分化。这些过程伴随着GAG含量的改变。这些结果表明，控制TGFbeta1的活性对于改善受损肌肉的再生和加速成肌细胞分化很重要，部分原因是通过改变肌肉细胞环境中的GAG组成。
