• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

壳聚糖和姜黄素纳米粒子对甘油损伤大鼠肌肉早期再生阶段骨骼肌纤维化的影响。

Effect of chitosan and curcumin nanoparticles against skeletal muscle fibrosis at early regenerative stage of glycerol-injured rat muscles.

机构信息

Department of Anatomy and Embryology, Faculty of Veterinary Medicine, South Valley University, Qena, 83523, Egypt.

Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy (Boys), Al-Azhar University, Nasr City, Cairo, Egypt.

出版信息

BMC Musculoskelet Disord. 2022 Jul 14;23(1):670. doi: 10.1186/s12891-022-05633-x.

DOI:10.1186/s12891-022-05633-x
PMID:35836166
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9281067/
Abstract

INTRODUCTION

Chitosan and curcumin are natural products that have a wide range of beneficial effects including wound healing. However, their high molecular weight and poor water solubility limit their applications.

AIMS

Therefore, the current study aims to evaluate the effects of chitosan (Cs) and curcumin (Cn) nanoparticles (NPs) on fibrosis and regeneration of glycerol-injured muscle.

METHODS

Muscle injury was induced by intramuscular injection of glycerol into the tibialis anterior muscle of rats. Cs-NPs and Cn-NPs were administered at different doses intraperitoneally after injury. Injured muscles were collected at day 7 after injury, and muscle fibrosis and regeneration were assessed.

RESULTS

The present results revealed that Cs-NPs and Cn-NPs treatment significantly decreased fibrosis index and increased the average myotube diameter with shifting of the distribution of myotube diameters towards larger diameters in a dose-dependent manner. Immunohistochemical analysis revealed that Cs-NPs and Cn-NPs treatment significantly decreased the number of CD-68 cells and Col-1 area. Results showed that Cn-NPs had a higher protective effect, in the form of attenuating muscle fibrosis and inflammation, and enhancing muscle regeneration, than that of Cs-NPs.

CONCLUSIONS

To our knowledge, this is the first study to document the effects of Cs-NPs in injured muscles. The results of study might be a novel approach to attenuate muscle fibrosis in humans using curcumin and chitosan nanoparticles.

摘要

简介

壳聚糖和姜黄素是具有广泛有益作用的天然产物，包括伤口愈合。然而，它们的高分子量和低水溶性限制了它们的应用。

目的

因此，本研究旨在评估壳聚糖（Cs）和姜黄素（Cn）纳米颗粒（NPs）对甘油损伤肌肉纤维化和再生的影响。

方法

通过向大鼠胫骨前肌肌内注射甘油诱导肌肉损伤。损伤后，通过腹腔内给予不同剂量的 Cs-NPs 和 Cn-NPs。损伤后 7 天采集损伤肌肉，评估肌肉纤维化和再生。

结果

本研究结果表明，Cs-NPs 和 Cn-NPs 治疗可显著降低纤维化指数，增加平均肌管直径，并以剂量依赖的方式将肌管直径的分布向较大直径转移。免疫组织化学分析表明，Cs-NPs 和 Cn-NPs 治疗可显著减少 CD-68 细胞和 Col-1 面积的数量。结果表明，Cn-NPs 在减轻肌肉纤维化和炎症、增强肌肉再生方面的保护作用高于 Cs-NPs。

结论

据我们所知，这是第一项研究记录 Cs-NPs 在损伤肌肉中的作用。该研究结果可能为使用姜黄素和壳聚糖纳米颗粒减轻人类肌肉纤维化提供一种新方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91a6/9281067/310a5289b142/12891_2022_5633_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91a6/9281067/ba39b7e1f142/12891_2022_5633_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91a6/9281067/fd96b39b6289/12891_2022_5633_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91a6/9281067/f93307f669e6/12891_2022_5633_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91a6/9281067/c50c13b08a23/12891_2022_5633_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91a6/9281067/7713b80977c2/12891_2022_5633_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91a6/9281067/e654ad8ecc48/12891_2022_5633_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91a6/9281067/310a5289b142/12891_2022_5633_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91a6/9281067/ba39b7e1f142/12891_2022_5633_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91a6/9281067/fd96b39b6289/12891_2022_5633_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91a6/9281067/f93307f669e6/12891_2022_5633_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91a6/9281067/c50c13b08a23/12891_2022_5633_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91a6/9281067/7713b80977c2/12891_2022_5633_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91a6/9281067/e654ad8ecc48/12891_2022_5633_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91a6/9281067/310a5289b142/12891_2022_5633_Fig7_HTML.jpg

相似文献

1
Effect of chitosan and curcumin nanoparticles against skeletal muscle fibrosis at early regenerative stage of glycerol-injured rat muscles.壳聚糖和姜黄素纳米粒子对甘油损伤大鼠肌肉早期再生阶段骨骼肌纤维化的影响。
BMC Musculoskelet Disord. 2022 Jul 14;23(1):670. doi: 10.1186/s12891-022-05633-x.
2
Effects of transforming growth factor-β1 treatment on muscle regeneration and adipogenesis in glycerol-injured muscle.转化生长因子-β1治疗对甘油损伤肌肉中肌肉再生和脂肪生成的影响。
Anim Sci J. 2017 Nov;88(11):1811-1819. doi: 10.1111/asj.12845. Epub 2017 Jun 6.
3
Polyelectrolyte Complex Nanoparticles from Chitosan and Acylated Rapeseed Cruciferin Protein for Curcumin Delivery.壳聚糖和酰化油菜籽芸苔球蛋白的聚电解质复合纳米粒子用于姜黄素传递。
J Agric Food Chem. 2018 Mar 21;66(11):2685-2693. doi: 10.1021/acs.jafc.7b05083. Epub 2018 Feb 27.
4
Comparative study of muscle regeneration following cardiotoxin and glycerol injury.心脏毒素和甘油损伤后肌肉再生的比较研究
Ann Anat. 2015 Nov;202:18-27. doi: 10.1016/j.aanat.2015.07.002. Epub 2015 Aug 21.
5
Neutralization of transforming growth factor (TGF)-β1 activity reduced fibrosis and enhanced regeneration of glycerol-injured rat muscle.转化生长因子（TGF）-β1活性的中和可减少甘油损伤大鼠肌肉的纤维化并增强其再生能力。
J Vet Med Sci. 2020 Feb 18;82(2):168-171. doi: 10.1292/jvms.19-0446. Epub 2019 Dec 25.
6
The role of the lecithin addition in the properties and cytotoxic activity of chitosan and chondroitin sulfate nanoparticles containing curcumin.添加卵磷脂对含姜黄素壳聚糖和硫酸软骨素纳米粒子性质和细胞毒性活性的作用。
Carbohydr Polym. 2020 Jan 1;227:115351. doi: 10.1016/j.carbpol.2019.115351. Epub 2019 Sep 21.
7
and Comparison of Curcumin-Encapsulated Chitosan-Coated Poly(lactic--glycolic acid) Nanoparticles and Curcumin/Hydroxypropyl-β-Cyclodextrin Inclusion Complexes Administered Intranasally as Therapeutic Strategies for Alzheimer's Disease.姜黄素包封壳聚糖-聚乳酸-羟基乙酸共聚物纳米粒子与姜黄素/羟丙基-β-环糊精包合物经鼻内给药治疗阿尔茨海默病的比较。
Mol Pharm. 2020 Nov 2;17(11):4256-4269. doi: 10.1021/acs.molpharmaceut.0c00675. Epub 2020 Oct 21.
8
-Acetyl-l-cysteine/l-Cysteine-Functionalized Chitosan-β-Lactoglobulin Self-Assembly Nanoparticles: A Promising Way for Oral Delivery of Hydrophilic and Hydrophobic Bioactive Compounds.乙酰-L-半胱氨酸/L-半胱氨酸功能化壳聚糖-β-乳球蛋白自组装纳米粒：一种用于口服传递亲水性和疏水性生物活性化合物的有前途的方法。
J Agric Food Chem. 2019 Nov 13;67(45):12511-12519. doi: 10.1021/acs.jafc.9b05219. Epub 2019 Nov 1.
9
Glycerol induces early fibrosis in regenerating rat skeletal muscle.甘油可诱导再生大鼠骨骼肌早期纤维化。
J Vet Med Sci. 2018 Nov 9;80(11):1646-1649. doi: 10.1292/jvms.18-0328. Epub 2018 Oct 2.
10
Synergistic Effects of Photo-Irradiation and Curcumin-Chitosan/Alginate Nanoparticles on Tumor Necrosis Factor-Alpha-Induced Psoriasis-Like Proliferation of Keratinocytes.光辐照和姜黄素壳聚糖/海藻酸钠纳米粒子对肿瘤坏死因子-α诱导的角质形成细胞银屑病样增殖的协同作用。
Molecules. 2019 Apr 9;24(7):1388. doi: 10.3390/molecules24071388.

引用本文的文献

1
Advancements in Drug Delivery Systems for the Treatment of Sarcopenia: An Updated Overview.药物输送系统治疗肌肉减少症的研究进展：最新综述。
Int J Mol Sci. 2024 Oct 7;25(19):10766. doi: 10.3390/ijms251910766.
2
Fibrosis and Sports Injuries: Concept and Implications: The GESMUTE-SETRADE Consensus Statement.纤维化与运动损伤：概念及影响：GESMUTE-SETRADE共识声明
Orthop J Sports Med. 2024 Sep 16;12(9):23259671241266604. doi: 10.1177/23259671241266604. eCollection 2024 Sep.
3
Curcumin-activated Wnt5a pathway mediates Ca channel opening to affect myoblast differentiation and skeletal muscle regeneration.

本文引用的文献

1
Toward Regulatory Effects of Curcumin on Transforming Growth Factor-Beta Across Different Diseases: A Review.姜黄素对不同疾病中转化生长因子-β的调节作用综述
Front Pharmacol. 2020 Dec 14;11:585413. doi: 10.3389/fphar.2020.585413. eCollection 2020.
2
Nanocurcumin: A Promising Candidate for Therapeutic Applications.纳米姜黄素：治疗应用的一个有前景的候选物。
Front Pharmacol. 2020 May 1;11:487. doi: 10.3389/fphar.2020.00487. eCollection 2020.
3
Chitosan based bioactive materials in tissue engineering applications-A review.基于壳聚糖的生物活性材料在组织工程中的应用——综述
姜黄素激活的Wnt5a信号通路介导钙通道开放，影响成肌细胞分化和骨骼肌再生。
J Cachexia Sarcopenia Muscle. 2024 Oct;15(5):1834-1849. doi: 10.1002/jcsm.13535. Epub 2024 Jul 10.
4
Curcumin Release from Biomaterials for Enhanced Tissue Regeneration Following Injury or Disease.用于损伤或疾病后增强组织再生的生物材料中姜黄素的释放
Bioengineering (Basel). 2023 Feb 16;10(2):262. doi: 10.3390/bioengineering10020262.
Bioact Mater. 2020 Feb 12;5(1):164-183. doi: 10.1016/j.bioactmat.2020.01.012. eCollection 2020 Mar.
4
Neutralization of transforming growth factor (TGF)-β1 activity reduced fibrosis and enhanced regeneration of glycerol-injured rat muscle.转化生长因子（TGF）-β1活性的中和可减少甘油损伤大鼠肌肉的纤维化并增强其再生能力。
J Vet Med Sci. 2020 Feb 18;82(2):168-171. doi: 10.1292/jvms.19-0446. Epub 2019 Dec 25.
5
Synthesis, Bioapplications, and Toxicity Evaluation of Chitosan-Based Nanoparticles.基于壳聚糖的纳米粒子的合成、生物应用和毒性评估。
Int J Mol Sci. 2019 Nov 16;20(22):5776. doi: 10.3390/ijms20225776.
6
Oral Administration of Chitosan Attenuates Bleomycin-induced Pulmonary Fibrosis in Rats.口服壳聚糖可减轻博来霉素诱导的大鼠肺纤维化。
In Vivo. 2019 Sep-Oct;33(5):1455-1461. doi: 10.21873/invivo.11624.
7
Curcumin and Nanocurcumin Oral Supplementation Improve Muscle Healing in a Rat Model of Surgical Muscle Laceration.姜黄素和纳米姜黄素口服补充剂可改善手术性肌肉撕裂大鼠模型中的肌肉愈合情况。
Bull Emerg Trauma. 2019 Jul;7(3):292-299. doi: 10.29252/beat-0703013.
8
Biomedical Applications of Chitosan and Its Derivative Nanoparticles.壳聚糖及其衍生物纳米颗粒的生物医学应用
Polymers (Basel). 2018 Apr 23;10(4):462. doi: 10.3390/polym10040462.
9
Effective Timing of Curcumin Ingestion to Attenuate Eccentric Exercise-Induced Muscle Soreness in Men.姜黄素摄入的有效时间对减轻男性离心运动引起的肌肉酸痛的影响
J Nutr Sci Vitaminol (Tokyo). 2019;65(1):82-89. doi: 10.3177/jnsv.65.82.
10
Skeletal muscle fibrosis: an overview.骨骼肌纤维化：概述。
Cell Tissue Res. 2019 Mar;375(3):575-588. doi: 10.1007/s00441-018-2955-2. Epub 2018 Nov 12.