Department of Biochemistry, University of Cambridge, UK.
Int J Mol Sci. 2009 Aug 4;10(8):3457-77. doi: 10.3390/ijms10083457.
Cooperative binding pervades Nature. This review discusses the use of isothermal titration calorimetry (ITC) in the identification and characterisation of cooperativity in biological interactions. ITC has broad scope in the analysis of cooperativity as it determines binding stiochiometries, affinities and thermodynamic parameters, including enthalpy and entropy in a single experiment. Examples from the literature are used to demonstrate the applicability of ITC in the characterisation of cooperative systems.
协同结合普遍存在于自然界中。本文综述了使用等温热力学滴定法（ITC）来鉴定和描述生物相互作用中的协同性。ITC 在分析协同性方面具有广泛的应用，因为它可以在单次实验中确定结合化学计量、亲和力和热力学参数，包括焓和熵。本文通过文献中的实例展示了 ITC 在描述协同体系中的适用性。
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